玻连蛋白在人胚胎干细胞神经发生过程中促进少突胶质细胞分化。
Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
作者信息
Gil Jung-Eun, Woo Dong-Hun, Shim Joong-Hyun, Kim Sung-Eun, You Hyun-Ju, Park Sung-Hye, Paek Sun Ha, Kim Suel-Kee, Kim Jong-Hoon
机构信息
Laboratory of Stem Cell Biology, Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
出版信息
FEBS Lett. 2009 Feb 4;583(3):561-7. doi: 10.1016/j.febslet.2008.12.061. Epub 2009 Jan 20.
We demonstrate enhanced differentiation of oligodendrocytes during neurogenesis of human embryonic stem cells (hESCs) using an extracellular matrix protein, vitronectin (VN). We show that VN is expressed in the ventral part of the developing human spinal cord. Combined treatment of retinoic acid, sonic hedgehog, and noggin in the presence of VN allows hESCs to differentiate into O4-positive oligodendrocytes. Particularly, VN profoundly promotes the derivation of oligodendrocyte progenitors that proliferate and differentiate into oligodendrocytes in response to mitogenic and survival factors. These results support the beneficial effect of VN on oligodendrocytic differentiation of hESCs.
我们证明，使用细胞外基质蛋白玻连蛋白（VN）可增强人胚胎干细胞（hESC）神经发生过程中少突胶质细胞的分化。我们发现VN在发育中的人脊髓腹侧表达。在VN存在的情况下，联合使用视黄酸、音猬因子和头蛋白处理可使hESC分化为O4阳性少突胶质细胞。特别是，VN能显著促进少突胶质前体细胞的产生，这些前体细胞在有丝分裂原和存活因子的作用下增殖并分化为少突胶质细胞。这些结果支持了VN对hESC少突胶质细胞分化的有益作用。
Zotero 插件