Kamiya S, Yamakawa K, Meng X Q, Ogura H, Nakamura S
Department of Bacteriology, School of Medicine, Kanazawa University, Japan.
FEMS Microbiol Lett. 1991 Jul 1;65(3):311-5. doi: 10.1016/0378-1097(91)90233-z.
Nine monoclonal antibodies (mAb) to Clostridium difficile toxin A were produced. The isotype of one mAb (37B5) was IgG2b, kappa, and that of the other eight mAbs was IgM, kappa. Immunoblot analysis after non-denatured PAGE showed that with the exception of one mAb (112G6) all mAbs gave a positive reaction with the 540 kDa band of toxin A. Immunoblot analysis showed that four mAbs (2E15, 3B4, 37B5 and 49C4) gave a positive reaction with the 240 kDa major band of toxin A. In neutralisation tests with these mAbs for enterotoxicity, mouse lethality, haemagglutination activity and cytotoxicity, 37B5 neutralised enterotoxicity in a rabbit ileal loop response test but did not neutralise any other biological activities. None of the other eight mAbs showed any neutralising activities at all.
制备了9种针对艰难梭菌毒素A的单克隆抗体（mAb）。其中一种单克隆抗体（37B5）的亚型为IgG2b、κ链，其他8种单克隆抗体的亚型为IgM、κ链。非变性聚丙烯酰胺凝胶电泳后的免疫印迹分析表明，除一种单克隆抗体（112G6）外，所有单克隆抗体均与毒素A的540 kDa条带呈阳性反应。免疫印迹分析表明，4种单克隆抗体（2E15、3B4、37B5和49C4）与毒素A的240 kDa主条带呈阳性反应。在用这些单克隆抗体进行的肠毒性、小鼠致死性、血凝活性和细胞毒性中和试验中，37B5在兔回肠袢反应试验中中和了肠毒性，但未中和任何其他生物学活性。其他8种单克隆抗体均未表现出任何中和活性。
