Costlow M E, McGuire W L
J Natl Cancer Inst. 1977 Apr;58(4):1173-5. doi: 10.1093/jnci/58.4.1173.
Prolactin receptors were localized by autoradiography in 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors from rats by incubation of tumor slices with [125I]ovine prolactin. Specific prolactin binding was confined to tumor cells, and nonspecific binding was present in alveolar spaces and connective tissue. In some tumors, all cells contained receptors; in others, up to one-half the cells remained unlabeled. These results suggested that variation in receptor content in DMBA-induced mammary tumors may be caused by two distinct populations of cells--one which contains receptors and another which possesses very few receptor sites or none at all.
通过将肿瘤切片与[125I]羊催乳素孵育，利用放射自显影技术在7,12 - 二甲基苯并[a]蒽（DMBA）诱导的大鼠乳腺肿瘤中定位催乳素受体。特异性催乳素结合局限于肿瘤细胞，非特异性结合存在于肺泡间隙和结缔组织中。在一些肿瘤中，所有细胞都含有受体；在另一些肿瘤中，多达一半的细胞未被标记。这些结果表明，DMBA诱导的乳腺肿瘤中受体含量的差异可能是由两种不同的细胞群体引起的——一种含有受体，另一种几乎没有或根本没有受体位点。
