使用双光子荧光显微镜在体外通过口腔牙龈检测龈下牙石。
Detection of subgingival calculus through oral gum in vitro using two-photon fluorescence microscopy.
作者信息
Tung Oi-Hong, Lee Shyh-Yuan, Lai Yu-Lin, Chen How-Foo
机构信息
School of Dentistry, National Yang Ming University, Taipei, Taiwan 112 ROC.
出版信息
Annu Int Conf IEEE Eng Med Biol Soc. 2008;2008:4051-4. doi: 10.1109/IEMBS.2008.4650099.
Periodontal disease is one of the main chronic infectious diseases of oral cavity and the principal cause of tooth loss in humans. The main cause of periodontal disease is the bacteria in calculus or plaque to cause the gum inflamed. Therefore detection and removal of subgingival calculus remains important to all periodontal therapy. However, subgingival calculus within the periodontal pocket remains a complicated and potentially delicate structure to be detected and treated with current dental armamentaria, namely dental x-rays and the dental probe. In this study, an innovative subgingival calculus detection system employing autofluorescence imaging has been developed. Feasibility of such a system on the detection of subgingival calculus is investigated in vitro. Autofluorescence imaging is based on two-photon time correlated fluorescence technique by a femtosecond pulsed Ti-sapphire laser.
牙周病是口腔主要的慢性感染性疾病之一，也是人类牙齿缺失的主要原因。牙周病的主要病因是牙结石或牙菌斑中的细菌导致牙龈发炎。因此，检测和清除龈下牙结石对所有牙周治疗都很重要。然而，牙周袋内的龈下牙结石仍然是一个复杂且可能很精细的结构，目前的牙科设备，即牙科X光和牙探针，难以对其进行检测和治疗。在本研究中，开发了一种采用自体荧光成像的创新型龈下牙结石检测系统。该系统在体外研究了其检测龈下牙结石的可行性。自体荧光成像基于飞秒脉冲钛宝石激光器的双光子时间相关荧光技术。
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