Nagayama J, Nagayama M, Wada K, Iida T, Hirakawa H, Matsueda T, Masuda Y
Laboratory of Environmental Medicine, School of Health Sciences, Kyushu University, Fukuoka, Japan.
Fukuoka Igaku Zasshi. 1991 May;82(5):221-7.
In this study, first, we investigated the effect of 7,8-benzoflavone (ANF), mitomycin C (MMC), a well-known genotoxic compound, and ANF plus MMC on the induction of sister chromatid exchanges (SCEs) in human whole-blood cultures. Second, we examined the effect of mixture of organochlorine compounds, which very resembled their contamination of healthy people in its composition, on the induction of SCEs in the same blood culture system in order to clarify their genotoxicity as a whole. The following results were obtained. 1. ANF and MMC significantly enhanced the number of SCEs/cell at the concentrations of 4 x 10(-5) M and 10(-8)M, respectively. When both of the compounds were simultaneously added in the blood cultures, their effects on the induction of SCEs seemed to be additive. 2. Without ANF in the blood culture system, namely, an usual system of the SCEs experiment, we could not find a dose-response relationship between the concentration of the mixture of organochlorine compounds and the induction of SCEs/cell. With ANF, however, we observed a fairly good dose-response relationship between them. 3. In the whole-blood culture system with ANF, we found significantly great number of SCEs/cell at the level of twenty times higher concentration of the organochlorine compounds than the ordinary level. According to the results described above and of our other studies, 50% effective concentration (EC50, about 2 SCEs/cell higher than control SCEs/cell) of the mixture was considered to be about 5 times greater level over the general one.(ABSTRACT TRUNCATED AT 250 WORDS)
在本研究中，首先，我们调查了7,8 - 苯并黄酮（ANF）、丝裂霉素C（MMC，一种著名的基因毒性化合物）以及ANF加MMC对人全血培养物中姐妹染色单体交换（SCE）诱导的影响。其次，我们检测了有机氯化合物混合物（其成分与健康人所受污染十分相似）对同一血液培养系统中SCE诱导的影响，以阐明其整体的基因毒性。得到了以下结果。1. ANF和MMC分别在浓度为4×10⁻⁵ M和10⁻⁸ M时显著增加了每个细胞的SCE数量。当两种化合物同时添加到血液培养物中时，它们对SCE诱导的作用似乎是相加的。2. 在血液培养系统中没有ANF时，即SCE实验的常规系统中，我们未发现有机氯化合物混合物浓度与每个细胞SCE诱导之间的剂量 - 反应关系。然而，加入ANF后，我们观察到它们之间有相当良好的剂量 - 反应关系。3. 在含有ANF的全血培养系统中，我们发现在有机氯化合物浓度比普通水平高20倍时，每个细胞的SCE数量显著增加。根据上述结果以及我们其他研究的结果，混合物的50%有效浓度（EC50，比对照SCE/细胞高约2个SCE/细胞）被认为比一般水平高约5倍。（摘要截短于250字）
