Corallo A, Savineau J P, Tricoche R, Foungbe S
Laboratoire de Pharmacologie, Faculté de Pharmacie, Université d'Abidjan, Ivory Coast.
Fundam Clin Pharmacol. 1991;5(4):319-29. doi: 10.1111/j.1472-8206.1991.tb00726.x.
The effects of the aqueous extract of leaves of Bridelia atroviridis (Bridelia), a small African tree, on the mechanical activity of rat uterus were studied. The aqueous extract of leaves of B atroviridis administered in a concentration-dependent manner (5 x 10(-6)-1.2 x 10(-3) g/ml) induced contractions that were antagonized by various calcium entry blockers (nifedipine, diltiazem, manganese chloride). In absence of external calcium ions, repeated applications of a supramaximal concentration of Bridelia (1.2 x 10(-3) g/ml) evoked sustained and repeated contractions the amplitude of which was congruent to 20% of those obtained in the physiological external calcium concentration. Bridelia-induced contractions in calcium-free medium were inhibited by isoprenaline (8 x 10(-7) M), caffeine (15 x 10(-3) M) and trifluoperazine (10(-5) M). Contractile responses induced by Bridelia in both calcium-containing and calcium-free media were antagonized by prior incubation of uterus with phorbol 12, 13-dibutyrate (6 x 10(-7) M), cholera toxin (6 x 10(-8) M) or pertussis toxin (5 x 10(-7) g/ml). These results show that Bridelia has a potent uterotonic action in the rat. The cellular basis of this action appears to be complex, and involves various mechanisms including calcium mobilization from both intra and extracellular compartments and activation of phospholipase C through a G-protein.
对非洲一种小型树木——黑绿土蜜树（Bridelia atroviridis）树叶水提取物对大鼠子宫机械活动的影响进行了研究。以浓度依赖性方式（5×10⁻⁶ - 1.2×10⁻³ g/ml）给予黑绿土蜜树树叶水提取物可诱导子宫收缩，多种钙通道阻滞剂（硝苯地平、地尔硫䓬、氯化锰）可拮抗这种收缩。在无细胞外钙离子的情况下，重复应用超最大浓度的黑绿土蜜树提取物（1.2×10⁻³ g/ml）可诱发持续且重复的收缩，其幅度相当于在生理细胞外钙浓度下所获收缩幅度的20%。异丙肾上腺素（8×10⁻⁷ M）、咖啡因（15×10⁻³ M）和三氟拉嗪（10⁻⁵ M）可抑制无钙培养基中黑绿土蜜树提取物诱导的收缩。在用佛波醇12,13 - 二丁酸酯（6×10⁻⁷ M）、霍乱毒素（6×10⁻⁸ M）或百日咳毒素（5×10⁻⁷ g/ml）预先孵育子宫后，黑绿土蜜树提取物在含钙和无钙培养基中诱导的收缩反应均被拮抗。这些结果表明，黑绿土蜜树对大鼠具有强大的子宫收缩作用。这种作用的细胞基础似乎很复杂，涉及多种机制，包括从细胞内和细胞外区室动员钙以及通过G蛋白激活磷脂酶C。
