Michelson Alan D
Center for Platelet Function Studies, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Am J Cardiol. 2009 Feb 2;103(3 Suppl):20A-26A. doi: 10.1016/j.amjcard.2008.11.019.
This article discusses the advantages and disadvantages of methods for the measurement of platelet function. The focus is on tests that can be used to monitor antiplatelet activity in the setting of cardiovascular disease and potentially predict thrombosis and bleeding. The tests described are platelet aggregometry; impedance aggregometry; VerifyNow (Accumetrics, San Diego, CA); Plateletworks (Helena Laboratories, Beaumont, TX); platelet surface P-selectin, platelet surface-activated glycoprotein IIb/IIIa, and leukocyte-platelet aggregates; TEG Platelet Mapping system (Haemoscope, Niles, IL); Impact cone and plate(let) analyzer (DiaMed, Cressier, Switzerland); Platelet Function Analyzer-100 (Siemens Healthcare Diagnostics, Inc., Deerfield, IL); phosphorylation of vasodilator-stimulated phosphoprotein; serum thromboxane B(2); and urinary 11-dehydro thromboxane B(2). Some of the factors that differentiate these tests are sample volume requirements, the use of whole blood, the presence of shear, point-of-care status, need for a technician, and expense.
本文讨论了血小板功能检测方法的优缺点。重点在于可用于监测心血管疾病背景下抗血小板活性并有可能预测血栓形成和出血的检测方法。所描述的检测方法包括血小板聚集测定法；阻抗聚集测定法；VerifyNow（Accumetrics公司，加利福尼亚州圣地亚哥）；Plateletworks（Helena Laboratories公司，得克萨斯州博蒙特）；血小板表面P-选择素、血小板表面活化糖蛋白IIb/IIIa以及白细胞-血小板聚集体；TEG血小板功能分析系统（Haemoscope公司，伊利诺伊州奈尔斯）；冲击圆锥平板（血小板）分析仪（DiaMed公司，瑞士克雷西耶）；血小板功能分析仪-100（西门子医疗诊断公司，伊利诺伊州迪尔菲尔德）；血管舒张刺激磷蛋白的磷酸化；血清血栓素B2；以及尿11-脱氢血栓素B2。区分这些检测方法的一些因素包括样本量要求、全血的使用、剪切力的存在、即时检测状态、对技术人员的需求以及费用。
