基于微过氧化物酶-11在多壁碳纳米管/室温离子液体纳米复合材料中的生物催化作用的生物传感器用于安培法测定过氧化氢。
Biosensor based on the biocatalysis of microperoxidase-11 in nanocomposite material of multiwalled carbon nanotubes/room temperature ionic liquid for amperometric determination of hydrogen peroxide.
作者信息
Wan Jun, Bi Jianli, Du Ping, Zhang Shusheng
机构信息
Key Laboratory of Ecochemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
出版信息
Anal Biochem. 2009 Mar 15;386(2):256-61. doi: 10.1016/j.ab.2008.12.028. Epub 2009 Jan 4.
A novel nanocomposite material of multiwalled carbon nanotubes (MWCNTs) and room temperature ionic liquid (RTIL) N-butylpyridinium hexafluorophosphate (BPPF6) was explored and used to construct a novel microperoxidase-11 (MP-11) biosensor for the determination of hydrogen peroxide (H2O2). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to characterize the performance of the biosensor. Under the optimized experimental conditions, H2O2 could be detected in a linear calibration range of 0.5 to 7.0 x 10(-7)mol L(-1) with a correlation coefficient of 0.9949 (n=9) and a detection limit of 3.8 x 10(-9) mol L(-1) at 3 sigma. The modified electrodes displayed excellent electrochemical response, high sensitivity, long-term stability, and good bioactivity and selectivity.
探索了一种由多壁碳纳米管（MWCNTs）和室温离子液体（RTIL）六氟磷酸正丁基吡啶鎓（BPPF6）组成的新型纳米复合材料，并将其用于构建一种新型的用于测定过氧化氢（H2O2）的微过氧化物酶-11（MP-11）生物传感器。采用循环伏安法（CV）和差分脉冲伏安法（DPV）对生物传感器的性能进行表征。在优化的实验条件下，H2O2的检测线性校准范围为0.5至7.0×10（-7）mol L（-1），相关系数为0.9949（n = 9），在3σ时检测限为3.8×10（-9）mol L（-1）。修饰电极表现出优异的电化学响应、高灵敏度、长期稳定性以及良好的生物活性和选择性。
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