Donna A, Betta P G, Bianchi V, Ribotta M, Bellingeri D, Robutti F, Marchesini A
Department of Pathology, City Hospital, Alessandria, Italy.
Histopathology. 1991 Sep;19(3):239-44. doi: 10.1111/j.1365-2559.1991.tb00028.x.
A myogenic phenotype was induced in cultures of human mesothelial cells treated for 72 h with atrazine, a triazine derivative. Immunoreactivity for both myosin and myoglobin was detected in a large number of these cells, irrespective of their polygonal or spindle morphology, whereas no expression of desmin was observed. These findings support the embryological identity of mesothelium and mesoderm, the former being, in the post-embryonic stage, potentially capable of differentiation along the same lineages which the latter normally displays during embryogenesis. In the light of this concept it can be assumed that primary malignancies arising from the mesothelium have the competence to express the pluripotent nature of embryonic mesoderm, and hence the term mesodermoma is appropriate for this group of tumours, including mesotheliomas in a classical sense. A postulated mechanism for the phenotypic change of mesothelial cells is also outlined, involving atrazine conversion to 5-aza-chloro-cytidine, a probable DNA hypomethylating and gene activating agent, like its analogue 5-azacytidine.
用三嗪衍生物莠去津处理人间皮细胞培养物72小时后，诱导出了肌源性表型。在大量这些细胞中检测到了肌球蛋白和肌红蛋白的免疫反应性，无论它们是多边形还是纺锤形形态，而未观察到结蛋白的表达。这些发现支持了间皮和中胚层的胚胎学同一性，前者在胚胎后阶段可能能够沿着后者在胚胎发生过程中通常显示的相同谱系进行分化。根据这一概念，可以假设起源于间皮的原发性恶性肿瘤有能力表达胚胎中胚层的多能性质，因此中胚层瘤这个术语适用于这一组肿瘤，包括经典意义上的间皮瘤。还概述了间皮细胞表型变化的一种假定机制，涉及莠去津转化为5-氮杂氯胞苷，一种可能的DNA去甲基化和基因激活剂，就像其类似物5-氮杂胞苷一样。
