Hojman Pernille, Taudorf Sarah, Lundby Carsten, Pedersen Bente Klarlund
Centre of Inflammation and Metabolism, Department of Infectious Diseases, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
Cytokine. 2009 Mar;45(3):154-7. doi: 10.1016/j.cyto.2008.12.005. Epub 2009 Jan 22.
Recent studies have shown that erythropoietin (EPO) offers protection against ischemia, hemorrhagic shock and systemic inflammation in many tissues and it has been suggested that EPO has anti-inflammatory effects. With the aim of investigating the potential acute anti-inflammatory effects of EPO in a human in vivo model of acute systemic low-grade inflammation, we measured circulating inflammatory mediators after intravenous administration of Escherichia coli endotoxin (LPS) bolus injection (0.1 ng/kg of body weight) in young healthy male subjects. The subjects were divided into three groups receiving either (1) LPS alone, (2) EPO alone (15,000 IE of rHuEPO) or (3) EPO and LPS. Endotoxin administration alone induced a 3-, 12- and 5-fold increase in plasma concentrations of TNF-alpha, IL-6 and IL-10, respectively, 3h after LPS challenge. When EPO was given prior to a bolus injection with endotoxin, the levels of TNF-alpha and IL-6 were enhanced by 5- and 40-fold, respectively, whereas the endotoxin-induced increase in IL-10 response was not influenced by EPO. In contrast to our hypothesis, we find that EPO augments the acute inflammatory effect.
最近的研究表明，促红细胞生成素（EPO）对许多组织的缺血、失血性休克和全身炎症具有保护作用，并且有人提出EPO具有抗炎作用。为了研究EPO在急性全身轻度炎症的人体体内模型中的潜在急性抗炎作用，我们在年轻健康男性受试者静脉注射大肠杆菌内毒素（LPS）推注（0.1 ng/kg体重）后测量了循环炎症介质。受试者分为三组，分别接受（1）单独LPS、（2）单独EPO（15,000国际单位重组人促红细胞生成素）或（3）EPO和LPS。单独给予内毒素在LPS攻击3小时后分别使血浆中TNF-α、IL-6和IL-10的浓度增加了3倍、12倍和5倍。当在推注内毒素之前给予EPO时，TNF-α和IL-6的水平分别提高了5倍和40倍，而内毒素诱导的IL-10反应增加不受EPO影响。与我们的假设相反，我们发现EPO增强了急性炎症作用。
