一种与可溶性环氧化物水解酶N端结构域同源的昆虫法尼基磷酸酶。
An insect farnesyl phosphatase homologous to the N-terminal domain of soluble epoxide hydrolase.
作者信息
Cao Li, Zhang Ping, Grant David F
机构信息
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA.
出版信息
Biochem Biophys Res Commun. 2009 Feb 27;380(1):188-92. doi: 10.1016/j.bbrc.2009.01.079. Epub 2009 Jan 23.
In insects, farnesyl pyrophosphate (FPP) is converted to juvenile hormone (JH) via a conserved pathway consisting of isoprenoid-derived metabolites. The first step of this pathway is presumed to be hydrolysis of FPP to farnesol in the ring gland. Based on alignment of putative phosphatases from Drosophila melanogaster with the phosphatase domain of soluble epoxide hydrolase, Phos2680 and Phos15739 with conserved phosphatase motifs were identified, cloned and purified. Both D. melanogaster phosphatases hydrolyzed para-nitrophenyl phosphate, however, Phos15739 also hydrolyzed FPP with a K(cat)/K(m) of 2.1x10(5)M(-1)s(-1). RT-PCR analysis revealed that Phos15739 was expressed in the ring gland and its expression was correlated with JHIII titer during development of D. melanogaster. N-acetyl-S-geranylgeranyl-l-cysteine was found to be a potent inhibitor of Phos15739 with an IC(50) value of 4.4muM. Thus, our data identify Phos15739 as a FPP phosphatase that likely catalyzes the hydrolysis of FPP to farnesol in D. melanogaster.
在昆虫中，法尼基焦磷酸（FPP）通过一条由类异戊二烯衍生代谢物组成的保守途径转化为保幼激素（JH）。该途径的第一步推测是在环腺中将FPP水解为法尼醇。基于黑腹果蝇推定磷酸酶与可溶性环氧化物水解酶磷酸酶结构域的比对，鉴定、克隆并纯化了具有保守磷酸酶基序的Phos2680和Phos15739。两种黑腹果蝇磷酸酶都能水解对硝基苯磷酸，然而，Phos15739也能水解FPP，其催化常数与米氏常数之比（K(cat)/K(m)）为2.1×10⁵M⁻¹s⁻¹。逆转录聚合酶链反应（RT-PCR）分析表明，Phos15739在环腺中表达，且其表达与黑腹果蝇发育过程中的JHIII滴度相关。发现N-乙酰-S-香叶基香叶基-L-半胱氨酸是Phos15739的有效抑制剂，半数抑制浓度（IC₅₀）值为4.4μM。因此，我们的数据确定Phos15739是一种FPP磷酸酶，可能在黑腹果蝇中催化FPP水解为法尼醇。
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