黑腹果蝇和哺乳动物胞质环氧化物水解酶对保幼激素III和保幼激素III双环氧物的体外代谢作用

In vitro metabolism of juvenile hormone III and juvenile hormone III bisepoxide by Drosophila melanogaster and mammalian cytosolic epoxide hydrolase.

作者信息

Casas J, Harshman L G, Messeguer A, Kuwano E, Hammock B D

机构信息

Department of Entomology, University of California, Davis 95616.

出版信息

Arch Biochem Biophys. 1991 Apr;286(1):153-8. doi: 10.1016/0003-9861(91)90021-a.

DOI:10.1016/0003-9861(91)90021-a

PMID:1910282

Abstract

In vitro metabolism of juvenile hormone III (JH III) and juvenile hormone III bisepoxide was investigated using purified mouse liver cytosolic epoxide hydrolase (cEH) and cell fractions from Drosophila melanogaster. JH III was metabolized faster than JH III bisepoxide by epoxide hydrolase activity in D. melanogaster cell fractions and by cEH. After incubation with JH III bisepoxide, all cell fractions and cEH produced epoxy-diol, cis- and trans-tetrahydrofuran-diols, and tetraol as metabolites. An increase in the concentration of cEH resulted in an increase in the proportion of tetraol as a JH III bisepoxide metabolite but this trend was not observed in the D. melanogaster cell fractions. Differences between cell fractions in the metabolism of JH III and JH III bisepoxide suggests the presence of juvenile hormone epoxide hydrolase isozymes.

摘要

利用纯化的小鼠肝脏胞质环氧化物水解酶（cEH）和黑腹果蝇的细胞组分，对保幼激素III（JH III）和保幼激素III双环氧物的体外代谢进行了研究。在黑腹果蝇细胞组分和cEH中，环氧水解酶活性使JH III的代谢速度比JH III双环氧物更快。与JH III双环氧物孵育后，所有细胞组分和cEH都产生了环氧二醇、顺式和反式四氢呋喃二醇以及四醇作为代谢产物。cEH浓度的增加导致四醇作为JH III双环氧物代谢产物的比例增加，但在黑腹果蝇细胞组分中未观察到这种趋势。JH III和JH III双环氧物在细胞组分代谢上的差异表明存在保幼激素环氧水解酶同工酶。