Shukla H, Gillespie G A, Srivastava R, Collins F, Chorney M J
Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
Genomics. 1991 Aug;10(4):905-14. doi: 10.1016/0888-7543(91)90178-h.
By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Q alpha-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Q alpha-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.
通过黏粒克隆、染色体步移和脉冲场凝胶电泳（PFGE）相结合的方法，我们对人类主要组织相容性复合体（MHC）的HLA - A亚区进行了精细定位。通过分离一个I类跳跃克隆，已将Qα样HLA - G I类基因定位在距HLA - H 100 kb范围内。用位点特异性探针与PFGE印迹杂交进一步支持了这些I类基因的紧密物理连锁关系。已发现上述两个I类基因均与HLA - A连锁，HLA - H距HLA - A基因不超过200 kb。这些数据支持了在人类MHC中可能存在一个由非经典HLA I类基因组成的Qα样亚区，该亚区在端粒方向上与HLA - A基因座相连。
