Kirszenbaum M, Moreau P, Gluckman E, Dausset J, Carosella E
Commissariat à l'Energie Atomique, Hôpital Saint-Louis Centre Hayem, Paris, France.
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4209-13. doi: 10.1073/pnas.91.10.4209.
The HLA-G monomorphic, nonclassical class I gene encodes the major histocompatibility complex (MHC) molecule, which is the only MHC antigen expressed on cytotrophoblast cells of placenta. In this work, we have investigated expression of the HLA-G gene in fetal tissues and adult peripheral blood cells by using a sensitive hot-start reverse transcriptase PCR technique. PCR amplification with HLA-G primers specific for exon 3 has enabled us to demonstrate an alternatively spliced form of HLA-G mRNA present in fetal first trimester trophoblasts and lacking exon 4 (HLA-G.3-5). This low abundance transcript (approximately 1:200) in comparison to full-length mRNA may encode the protein that excludes the alpha 3 domain and by conformational changes may present a different ability to bind to peptides. Moreover, expression of the HLA-G transcript was found in adult peripheral lymphocytes and equally in B- and T-cell populations. These results are discussed in the context of the fetal-maternal relationship presented by HLA-G gene products.
HLA - G单态性非经典I类基因编码主要组织相容性复合体（MHC）分子，该分子是胎盘细胞滋养层细胞上表达的唯一MHC抗原。在本研究中，我们通过使用灵敏的热启动逆转录酶PCR技术，研究了HLA - G基因在胎儿组织和成人外周血细胞中的表达。用针对外显子3的HLA - G引物进行PCR扩增，使我们能够证明在孕早期胎儿滋养层细胞中存在一种选择性剪接形式的HLA - G mRNA，其缺少外显子4（HLA - G.3 - 5）。与全长mRNA相比，这种低丰度转录本（约为1:200）可能编码排除α3结构域的蛋白质，并且通过构象变化可能具有不同的结合肽的能力。此外，在成人外周淋巴细胞以及B细胞和T细胞群体中均发现了HLA - G转录本的表达。我们将结合HLA - G基因产物所呈现的胎儿 - 母体关系来讨论这些结果。
