Nagabhushan M, Sarode A V, Nair J, Amonkar A J, D'Souza A V, Bhide S V
Carcinogenesis Division, Cancer Research Institute, Bombay, India.
Indian J Exp Biol. 1991 May;29(5):401-6.
Aqueous, caffeine free and tannin fractions of commercial tea and tannic acid were tested for mutagenicity in Ames test. Tea fractions of tannic acid were non mutagenic in strains TA 100, TA 98, TA 1535 and TA 1538 of Salmonella typhimurium with or without metabolic activation (rat-S9 mix) at different doses tested. In strain TA 98 the above tea fractions and tannic acid inhibited the S9 mix mediated mutagenicity of tobacco in a dose dependent manner. The different tea fractions at 60 degrees C, did not increase the tumor incidence in Swiss mice by gavage feeding. They also failed to produce tumors when injected subcutaneously. Caffeine free tea extract decreased the tobacco induced liver tumors but had no effect on lung tumors. The same fraction was ineffective in hexachlorocyclohexane induced liver tumors in Swiss mice.
对市售茶叶的水相、无咖啡因和单宁组分以及单宁酸进行了艾姆斯试验中的致突变性测试。单宁酸的茶叶组分在不同剂量下，无论有无代谢活化（大鼠-S9混合物），在鼠伤寒沙门氏菌的TA 100、TA 98、TA 1535和TA 1538菌株中均无致突变性。在TA 98菌株中，上述茶叶组分和单宁酸以剂量依赖的方式抑制了烟草的S9混合物介导的致突变性。60摄氏度下的不同茶叶组分通过灌胃喂养未增加瑞士小鼠的肿瘤发生率。皮下注射时它们也未能产生肿瘤。无咖啡因的茶提取物减少了烟草诱导的肝肿瘤，但对肺肿瘤没有影响。相同组分对瑞士小鼠六氯环己烷诱导的肝肿瘤无效。
