Kon Y, Hashimoto Y, Kitagawa H, Sugimura M, Murakami K
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Anat. 1991 Jun;176:23-33.
Renin-containing cells in fetal adrenal glands of the mouse were investigated with the protein A-gold immunocytochemical technique. On Day 14 of gestation, a small number of specific granules were weakly immunoreactive and were distributed in the Golgi region. Sometimes, apparent exocytosis of gold-labelled particles could be seen opening into the extracellular space. On Day 16 of gestation, numerous gold particles were demonstrated in the Golgi region as well as in the specific secretory granules. Immunoreactivity of the specific granules was increased as compared with Day 14, though some granules were observed to have no reaction with the antibody. On Day 18 of gestation reactivity for renin decreased, while a few clustered immunoreactive granules were demonstrated just beneath the cell membrane. No gold particles were observed in the Golgi apparatus during this period and more granules negative for renin were noted than on Day 16 of gestation. These results suggest that renin is produced and released temporarily by adrenal cortical cells in the late fetal life of the mouse.
用蛋白A - 金免疫细胞化学技术研究了小鼠胎儿肾上腺中含肾素的细胞。在妊娠第14天，少量特异性颗粒免疫反应较弱，分布在高尔基体区域。有时可见明显的金标记颗粒胞吐作用通向细胞外空间。在妊娠第16天，高尔基体区域以及特异性分泌颗粒中可见大量金颗粒。与第14天相比，特异性颗粒的免疫反应性增强，尽管观察到一些颗粒与抗体无反应。在妊娠第18天，肾素反应性降低，而在细胞膜下方可见一些聚集的免疫反应性颗粒。在此期间高尔基体中未观察到金颗粒，且与妊娠第16天相比，肾素阴性的颗粒更多。这些结果表明，肾素在小鼠胎儿后期由肾上腺皮质细胞暂时产生和释放。
