葡萄糖转运蛋白1在犬骨肉瘤中的表达

Glucose transporter 1 expression in canine osteosarcoma.

作者信息

Petty J C, Lana S E, Thamm D H, Charles J B, Bachand A M, Bush J M, Ehrhart E J

机构信息

Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1678, USA.

出版信息

Vet Comp Oncol. 2008 Jun;6(2):133-40. doi: 10.1111/j.1476-5829.2007.00155.x.

DOI:10.1111/j.1476-5829.2007.00155.x

PMID:19178673

Abstract

Hypoxia in tumours has been associated with an increased resistance to radiation and chemotherapy, and increased metastatic potential. Hypoxia-inducible factor 1-alpha (HIF-1alpha) is a transcription factor induced by hypoxia. Glucose transporter 1 (GLUT-1), a downstream product of HIF-1alpha pathway activation, is over-expressed in a variety of human tumours. The purpose of this study was to determine if GLUT-1 is expressed in canine osteosarcomas (OSAs) and if the expression is related to tumour necrosis and outcome. Immunohistochemistry was performed on 44 histologically confirmed OSA tissue samples to assess expression of GLUT-1. Of 44 cases, 27 (61%) expressed GLUT-1. There was no statistical correlation between GLUT-1 and disease-free interval, survival time or percentage of necrosis. As hypothesized, GLUT-1 is present in canine appendicular OSAs. A more objective evaluation of GLUT-1 and other proteins in the HIF-1alpha pathway may be warranted.

摘要

肿瘤中的缺氧与对放疗和化疗的抗性增加以及转移潜能增加有关。缺氧诱导因子1-α（HIF-1α）是一种由缺氧诱导的转录因子。葡萄糖转运蛋白1（GLUT-1）是HIF-1α途径激活的下游产物，在多种人类肿瘤中过度表达。本研究的目的是确定GLUT-1是否在犬骨肉瘤（OSA）中表达，以及该表达是否与肿瘤坏死和预后相关。对44个经组织学确诊的OSA组织样本进行免疫组织化学检测，以评估GLUT-1的表达。在44例病例中，27例（61%）表达GLUT-1。GLUT-1与无病间期、生存时间或坏死百分比之间无统计学相关性。如所假设的，GLUT-1存在于犬肢体OSA中。可能需要对HIF-1α途径中的GLUT-1和其他蛋白质进行更客观的评估。