[陕西省部分地区零售肉类中沙门氏菌耐药性检测与分析]

[Detection and analysis of antibiotic resistance of Salmonella from retail meats in some districts of Shaanxi province].

作者信息

Shen Jin-ling, Yang Bao-wei, Zhi Shuai, Cui Sheng-hui, Xi Mei-li, Yang Peng-fei, Meng Jiang-hong

机构信息

College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling Shaanxi 712100, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2008 Oct;42(10):758-61.

PMID:19178840

Abstract

OBJECTIVE

Salmonella isolates recovered from retail meats that were collected in supermarkets and free markets in Xi'an and Yangling areas of Shaanxi province were studied to determine antibiotic susceptibility.

METHOD

Antimicrobial susceptibility to 14 antibiotics of 193 salmonella isolates were determined by using agar dilution method, which was recommended by National Committee of Clinical Laboratory Standard (NCCLS), and E.coli ATCC25922 and E.faecalis ATCC29212 as standard control strains.

RESULTS

The 44.6% of the salmonella isolates were resistant to sulfamethoxazole, followed by resistance to kanamycin (40.9%), tetracycline (37.8%), amoxicillin (26.9%), ampicillin (25.4%), gentamicin (23.3%) and chloramphenicol (21.8%). Some isolates also showed resistance to fluoroquinolones, the rates for ciprofloxacin, enrofloxacin, levofloxacin and gatifloxacin were 22.3%, 21.8%, 20.8% and 21.2%, respectively. 55 isolates (28.5%) were multidrug resistant (MDR) strains, 28 of 193 isolates (14.5%) could resist at least 13 antibiotics, 24 isolates (12.4%) were resistant to from 4 to 12 antibiotics.

CONCLUSION

Salmonella isolates recovered from retail meats in Xi'an district of Shaanxi province were seriously resistant to antimicrobials commonly used as human and veterinary medicine.

摘要

目的

对从陕西省西安和杨凌地区超市及自由市场采集的零售肉类中分离出的沙门氏菌进行研究，以确定其抗生素敏感性。

方法

采用美国国家临床实验室标准委员会（NCCLS）推荐的琼脂稀释法，以大肠埃希菌ATCC25922和粪肠球菌ATCC29212作为标准对照菌株，测定193株沙门氏菌对14种抗生素的敏感性。

结果

44.6%的沙门氏菌分离株对磺胺甲恶唑耐药，其次是对卡那霉素（40.9%）、四环素（37.8%）、阿莫西林（26.9%）、氨苄西林（25.4%）、庆大霉素（23.3%）和氯霉素（21.8%）耐药。部分分离株还对氟喹诺酮类耐药，环丙沙星、恩诺沙星、左氧氟沙星和加替沙星的耐药率分别为22.3%、21.8%、20.8%和21.2%。55株（28.5%）为多重耐药（MDR）菌株，193株中有28株（14.5%）可耐受至少13种抗生素，24株（12.4%）对4至12种抗生素耐药。