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巴西新热带寡毛实蝇(Nyssomyia neivai)(双翅目:蚤蝇科,血蝇亚科)自然感染利什曼原虫(Viannia)种属。

Natural infection of Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae, Phlebotominae) by Leishmania (Viannia) spp. in Brazil.

机构信息

Department of Microbiology and Parasitology, Federal University of Santa Catarina, 88040-900 Florianópolis, Santa Catarina, Brazil.

出版信息

Trans R Soc Trop Med Hyg. 2009 Nov;103(11):1093-7. doi: 10.1016/j.trstmh.2008.12.006. Epub 2009 Jan 28.

Abstract

A study of the natural infection of phlebotomine sand flies by Leishmania (Viannia) was conducted in a focus of cutaneous leishmaniasis in Piçarras, on the northeastern coast of the Brazilian state of Santa Catarina. In total, 562 female Nyssomyia neivai were collected by miniature light traps near houses, separated in 61 pools and examined by PCR and Southern blot hybridization. Eight pools, four of them from the same light trap/night, were positive. This is the first finding of natural infection by Le. braziliensis of adequately identified Ny. neivai in Brazil. In this preliminary observation we observed the abundance and predominance of Ny. neivai among the captured phlebotomine species (98.5%), indicating that Ny. neivai may be the dominant vector of Leishmania in the subgenus Viannia in this area.

摘要

在巴西圣卡塔琳娜州东北部皮沙拉斯的皮肤利什曼病流行区,对致乏库蠓沙蝇的利什曼原虫(Viannia)自然感染进行了研究。共使用小型诱蚊灯在房屋附近采集了 562 只雌性 Nyssomyia neivai,将其分为 61 个池,并通过 PCR 和 Southern blot 杂交进行检查。有 8 个池(其中 4 个来自同一诱蚊灯/夜)呈阳性。这是在巴西首次发现自然感染的 Le. braziliensis 的 Ny. neivai。在这一初步观察中,我们观察到在捕获的致乏库蠓沙蝇物种中,Ny. neivai 的丰度和优势(98.5%),表明在该地区,Ny. neivai 可能是 Viannia 亚属中利什曼原虫的主要传播媒介。

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