Li Chong-jian, Gao Run-lin, Yang Yue-jin, Hu Feng-huan, Yang Wei-xian, You Shi-jie, Song Lai-feng, Ruan Ying-mao, Qiao Shu-bin, Chen Ji-lin, Li Jian-jun
Center of Coronary Heart Disease, Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037.
Chin Med Sci J. 2008 Dec;23(4):234-8. doi: 10.1016/s1001-9294(09)60045-6.
To investigate the therapeutic effectiveness of intracoronary implantation of autologous bone marrow mononuclear cells (BM-MNC) in miniswine model of reperfused myocardial infarction.
Sixteen miniswine myocardial ischemic reperfusion injury models made by ligation of the distal one third segment of left anterior descending artery for 90 minutes were randomized into 2 groups. In BM-MNC group (n = 9), (3.54 +/- 0.90) X 10(8) BM-MNC were intracoronary injected, and in the control group (n = 7), phosphate buffered saline was injected by the same way. Echocardiographic and hemodynamic results, vessel density, and myocardial infarction size were evaluated and compared before and 4 weeks after cell transplantation.
In BM-MNC group, there were no differences between before and 4 weeks after transplantation in aspects of left ventricular ejection fraction (LVEF), interventricular septal thickness, left ventricular lateral and anterior septal wall thickness, cardiac output, or +dp/dtmax. In control group, LVEF, interventricular septal thickness, left ventricular lateral and anterior septal wall thickness, cardiac output, and +dp/dtmax decreased significantly 4 weeks after transplantation (P < 0.05). Left ventricular end-diastolic pressure and -dp/dtmax, did not change significantly before and after cell transplantation in both groups. Capillary density in BM-MNC group was greater than that in control group [(13.39 +/- 6.96)/high power field vs. (3.50 +/- 1.90)/high power field, P < 0.05]. Infarction area assessed by tetrazolium red staining and the infarction percentage decreased in BM-MNC group compared with those in control group (P < 0.05).
Transplantation of BM-MNC into myocardium with ischemic reperfusion injury increases capillary density and decreases infarction area. It has significantly beneficial effect on cardiac systolic function rather than on diastolic function.
研究自体骨髓单个核细胞（BM-MNC）冠状动脉内植入对小型猪再灌注心肌梗死模型的治疗效果。
将16只通过结扎左前降支远端三分之一段90分钟制成的小型猪心肌缺血再灌注损伤模型随机分为2组。BM-MNC组（n = 9）冠状动脉内注射（3.54±0.90）×10⁸个BM-MNC，对照组（n = 7）以同样方式注射磷酸盐缓冲液。在细胞移植前及移植后4周评估并比较超声心动图和血流动力学结果、血管密度及心肌梗死面积。
BM-MNC组移植前与移植后4周在左心室射血分数（LVEF）、室间隔厚度、左心室侧壁和前间隔壁厚度、心输出量或 +dp/dtmax方面无差异。对照组移植后4周LVEF、室间隔厚度、左心室侧壁和前间隔壁厚度、心输出量及 +dp/dtmax显著降低（P < 0.05）。两组细胞移植前后左心室舒张末期压力及 -dp/dtmax均无显著变化。BM-MNC组毛细血管密度大于对照组[（13.39±6.96）/高倍视野 vs.（3.50±1.90）/高倍视野，P < 0.05]。与对照组相比，BM-MNC组经四氮唑红染色评估的梗死面积及梗死百分比降低（P < 0.05）。
将BM-MNC移植到缺血再灌注损伤心肌可增加毛细血管密度并减小梗死面积。对心脏收缩功能有显著有益作用，而非对舒张功能。
