Rab1b调节内质网与后续高尔基体区室之间的囊泡运输。
Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
作者信息
Plutner H, Cox A D, Pind S, Khosravi-Far R, Bourne J R, Schwaninger R, Der C J, Balch W E
机构信息
Department of Molecular and Cellular Biology, Scripps Research Institute, La Jolla, California 92037.
出版信息
J Cell Biol. 1991 Oct;115(1):31-43. doi: 10.1083/jcb.115.1.31.
Abstract
We report an essential role for the ras-related small GTP-binding protein rab1b in vesicular transport in mammalian cells. mAbs detect rab1b in both the ER and Golgi compartments. Using an assay which reconstitutes transport between the ER and the cis-Golgi compartment, we find that rab1b is required during an initial step in export of protein from the ER. In addition, it is also required for transport of protein between successive cis- and medial-Golgi compartments. We suggest that rab1b may provide a common link between upstream and downstream components of the vesicular fission and fusion machinery functioning in early compartments of the secretory pathway.
摘要
我们报道了一种与Ras相关的小GTP结合蛋白rab1b在哺乳动物细胞囊泡运输中发挥的重要作用。单克隆抗体可在ER和高尔基体区室中检测到rab1b。通过一种重建ER与顺式高尔基体区室之间运输的检测方法，我们发现在蛋白质从ER输出的初始步骤中需要rab1b。此外，在顺式高尔基体和中间高尔基体区室之间的蛋白质运输中也需要它。我们认为rab1b可能在分泌途径早期区室中发挥作用的囊泡裂变和融合机制的上游和下游组分之间提供了一个共同的联系。
相似文献
1
Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.Rab1b调节内质网与后续高尔基体区室之间的囊泡运输。
J Cell Biol. 1991 Oct;115(1):31-43. doi: 10.1083/jcb.115.1.31.
2
Multiple GTP-binding proteins regulate vesicular transport from the ER to Golgi membranes.多种GTP结合蛋白调节从内质网到高尔基体膜的囊泡运输。
J Cell Biol. 1992 Dec;119(5):1077-96. doi: 10.1083/jcb.119.5.1077.
3
GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex.rab1和rab2的GTP结合突变体是内质网到高尔基体复合体囊泡运输的有效抑制剂。
J Cell Biol. 1992 Nov;119(4):749-61. doi: 10.1083/jcb.119.4.749.
4
Guanine nucleotide dissociation inhibitor is essential for Rab1 function in budding from the endoplasmic reticulum and transport through the Golgi stack.鸟嘌呤核苷酸解离抑制剂对于Rab1在内质网出芽及通过高尔基体堆叠运输过程中的功能至关重要。
J Cell Biol. 1994 Sep;126(6):1393-406. doi: 10.1083/jcb.126.6.1393.
5
A GDP-bound of rab1 inhibits protein export from the endoplasmic reticulum and transport between Golgi compartments.与GDP结合的rab1抑制蛋白质从内质网输出以及在高尔基体各间隔之间的转运。
J Cell Biol. 1994 Apr;125(2):225-37. doi: 10.1083/jcb.125.2.225.
6
Differential inhibition of multiple vesicular transport steps between the endoplasmic reticulum and trans Golgi network.内质网与反式高尔基体网络之间多个囊泡运输步骤的差异性抑制
J Biol Chem. 1993 Feb 25;268(6):4216-26.
7
Beta-COP is essential for transport of protein from the endoplasmic reticulum to the Golgi in vitro.β-COP对于体外蛋白质从内质网到高尔基体的转运至关重要。
J Cell Biol. 1993 Sep;122(6):1155-67. doi: 10.1083/jcb.122.6.1155.
8
A Rab2 mutant with impaired GTPase activity stimulates vesicle formation from pre-Golgi intermediates.一种具有受损GTP酶活性的Rab2突变体刺激来自高尔基前体中间产物的囊泡形成。
Mol Biol Cell. 1999 Jun;10(6):1837-49. doi: 10.1091/mbc.10.6.1837.
9
Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments.Sar1促进内质网而非高尔基体区室的囊泡出芽。
J Cell Biol. 1994 Apr;125(1):51-65. doi: 10.1083/jcb.125.1.51.
10
Characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the RER to the Golgi complex requires only one vesicular transport step.小鼠肝炎病毒出芽区室的特征：从内质网到高尔基体复合体的转运仅需一个囊泡转运步骤的证据。
J Cell Biol. 1994 Jan;124(1-2):55-70. doi: 10.1083/jcb.124.1.55.
引用本文的文献
1
An RNAi screen of Rab GTPase genes in Caenorhabditis elegans reveals that morphogenesis has a higher demand than stem cell niche maintenance for rab-1 in the somatic cells of the reproductive system.在秀丽隐杆线虫中对Rab GTPase基因进行的RNA干扰筛选表明，在生殖系统的体细胞中，形态发生对rab-1的需求高于干细胞微环境维持。
G3 (Bethesda). 2025 Jun 4;15(6). doi: 10.1093/g3journal/jkaf085.
2
An RNAi screen of Rab GTPase genes in reveals that somatic cells of the reproductive system depend on for morphogenesis but not stem cell niche maintenance.对Rab GTP酶基因进行的RNA干扰筛选表明，生殖系统的体细胞在形态发生方面依赖于 ，但在干细胞微环境维持方面并非如此。 （注：原文中“reveals that somatic cells of the reproductive system depend on for morphogenesis but not stem cell niche maintenance.”部分有缺失信息，此处翻译按原文呈现）
bioRxiv. 2024 Dec 6:2024.12.03.626641. doi: 10.1101/2024.12.03.626641.
3
Movement of the endoplasmic reticulum is driven by multiple classes of vesicles marked by Rab-GTPases.内质网的移动由以Rab-GTP酶为标记的多种囊泡驱动。
Mol Biol Cell. 2025 Jan 1;36(1):ar9. doi: 10.1091/mbc.E24-04-0197. Epub 2024 Dec 4.
4
Physiological and differential protein expression analyses of the calcium stress response in the rhizome.根茎中钙胁迫响应的生理及差异蛋白表达分析
Heliyon. 2024 Sep 21;10(19):e38260. doi: 10.1016/j.heliyon.2024.e38260. eCollection 2024 Oct 15.
5
A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion.内质网高尔基复合体中间膜蛋白 10 介导的非典型蛋白分泌过程中内质网高尔基复合体定位 Rab 蛋白的双重作用
Nat Cell Biol. 2024 Jul;26(7):1077-1092. doi: 10.1038/s41556-024-01445-4. Epub 2024 Jun 26.
6
-containing vacuoles interact with host recycling endosomal proteins Rab11a and Rab35 for vacuolar expansion and bacterial growth.-含有空泡的 vacuoles 与宿主再循环内体蛋白 Rab11a 和 Rab35 相互作用，导致空泡扩张和细菌生长。
Front Cell Infect Microbiol. 2024 May 22;14:1394019. doi: 10.3389/fcimb.2024.1394019. eCollection 2024.
7
A guide to selecting high-performing antibodies for Rab1A and Rab1B for use in Western Blot, immunoprecipitation and immunofluorescence.用于 Western Blot、免疫沉淀和免疫荧光的 Rab1A 和 Rab1B 高活性抗体选择指南。
F1000Res. 2025 Mar 7;12:1578. doi: 10.12688/f1000research.143928.2. eCollection 2023.
8
Cellular Release of Infectious Hepatitis C Virus Particles via Endosomal Pathways.细胞通过内体途径释放感染性丙型肝炎病毒颗粒。
Viruses. 2023 Dec 14;15(12):2430. doi: 10.3390/v15122430.
9
Transcriptomic and metabolomic analyses reveal the essential nature of Rab1B in Toxoplasma gondii.转录组学和代谢组学分析揭示 Rab1B 在弓形虫中的重要特性。
Parasit Vectors. 2023 Nov 8;16(1):409. doi: 10.1186/s13071-023-06030-6.
10
Functional screening reveals prenylated proteins required for endocytic trafficking and rhoptry protein sorting.功能筛选揭示了参与内吞运输和棒状体蛋白分拣的 prenylated 蛋白。
mBio. 2023 Aug 31;14(4):e0130923. doi: 10.1128/mbio.01309-23. Epub 2023 Aug 7.
本文引用的文献
1
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.酵母分泌途径中翻译后事件所需的23个互补组的鉴定。
Cell. 1980 Aug;21(1):205-15. doi: 10.1016/0092-8674(80)90128-2.
2
Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.通过 N-乙酰葡糖胺的偶联掺入来测定高尔基体连续区室之间蛋白质转运的重建。
Cell. 1984 Dec;39(2 Pt 1):405-16. doi: 10.1016/0092-8674(84)90019-9.
3
Pre- and post-Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface.高尔基体前和高尔基体后液泡在将塞姆利基森林病毒膜糖蛋白转运到细胞表面的过程中发挥作用。
Cell. 1984 Sep;38(2):535-49. doi: 10.1016/0092-8674(84)90508-7.
4
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
Envelope proteins of vesicular stomatitis virus: effect of temperature-sensitive mutations in complementation groups III and V.水疱性口炎病毒的包膜蛋白：互补群III和V中温度敏感突变的影响
J Virol. 1974 Nov;14(5):1220-8. doi: 10.1128/JVI.14.5.1220-1228.1974.
6
Subcellular fractionation of rat liver.大鼠肝脏的亚细胞分级分离
Methods Enzymol. 1974;31:6-41. doi: 10.1016/0076-6879(74)31005-1.
7
A ras-like protein is required for a post-Golgi event in yeast secretion.酵母分泌过程中高尔基体后事件需要一种类Ras蛋白。
Cell. 1987 May 22;49(4):527-38. doi: 10.1016/0092-8674(87)90455-7.
8
Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells.外分泌胰腺细胞中分泌蛋白通过高尔基体复合体运输过程中的温度敏感步骤。
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6425-9. doi: 10.1073/pnas.83.17.6425.
9
Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library.通过寡核苷酸策略分离出的ras基因超家族的另外四个成员：从大鼠脑文库中克隆YPT相关cDNA
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8210-4. doi: 10.1073/pnas.84.23.8210.
10
ras genes.RAS基因
Annu Rev Biochem. 1987;56:779-827. doi: 10.1146/annurev.bi.56.070187.004023.
Zotero 插件