Plutner H, Cox A D, Pind S, Khosravi-Far R, Bourne J R, Schwaninger R, Der C J, Balch W E
Department of Molecular and Cellular Biology, Scripps Research Institute, La Jolla, California 92037.
J Cell Biol. 1991 Oct;115(1):31-43. doi: 10.1083/jcb.115.1.31.
We report an essential role for the ras-related small GTP-binding protein rab1b in vesicular transport in mammalian cells. mAbs detect rab1b in both the ER and Golgi compartments. Using an assay which reconstitutes transport between the ER and the cis-Golgi compartment, we find that rab1b is required during an initial step in export of protein from the ER. In addition, it is also required for transport of protein between successive cis- and medial-Golgi compartments. We suggest that rab1b may provide a common link between upstream and downstream components of the vesicular fission and fusion machinery functioning in early compartments of the secretory pathway.
我们报道了一种与Ras相关的小GTP结合蛋白rab1b在哺乳动物细胞囊泡运输中发挥的重要作用。单克隆抗体可在ER和高尔基体区室中检测到rab1b。通过一种重建ER与顺式高尔基体区室之间运输的检测方法，我们发现在蛋白质从ER输出的初始步骤中需要rab1b。此外，在顺式高尔基体和中间高尔基体区室之间的蛋白质运输中也需要它。我们认为rab1b可能在分泌途径早期区室中发挥作用的囊泡裂变和融合机制的上游和下游组分之间提供了一个共同的联系。
