Lewintre Eloisa Jantus, Martín Cristina Reinoso, Ballesteros Carlos García, Montaner David, Rivera Rosa Farrás, Mayans José Ramón, García-Conde Javier
Molecular Haematology Laboratory, Prince Felipe Research Centre, Valencia, Spain.
Haematologica. 2009 Feb;94(2):280-4. doi: 10.3324/haematol.13052.
Chronic lymphocytic leukemia is an adult-onset leukemia with a heterogeneous clinical behavior. When chronic lymphocytic leukemia cases were divided on the basis of IgV(H) mutational status, widely differing clinical courses were revealed. Since IgV(H) sequencing is difficult to perform in a routine diagnostic laboratory, finding a surrogate for IgV(H) mutational status seems an important priority. In the present study, we proposed the use of Cryptochrome-1 as a new prognostic marker in early-stage chronic lymphocytic leukemia. Seventy patients (Binet stage A, without treatment) were included in the study. We correlated Cryptochrome-1 mRNA with well established prognostic markers such as IgV(H) mutations, ZAP70, LPL or CD38 expression and chromosomal abnormalities. High Cryptochrome-1 expression correlated with IgV(H) unmutated samples. In addition, Cryptochrome-1 was a valuable predictor of disease progression in early-stage chronic lymphocytic leukemia, therefore it can be introduced in clinical practice with the advantage of a simplified method of quantification.
慢性淋巴细胞白血病是一种成人发病的白血病，具有异质性的临床行为。当根据IgV(H)突变状态对慢性淋巴细胞白血病病例进行划分时，发现了差异很大的临床病程。由于在常规诊断实验室中难以进行IgV(H)测序，寻找IgV(H)突变状态的替代指标似乎是一个重要的优先事项。在本研究中，我们提议将隐花色素-1用作早期慢性淋巴细胞白血病的一种新的预后标志物。该研究纳入了70例患者（Binet A期，未接受治疗）。我们将隐花色素-1 mRNA与已确立的预后标志物如IgV(H)突变、ZAP70、LPL或CD38表达以及染色体异常进行了关联分析。隐花色素-1高表达与IgV(H)未突变样本相关。此外，隐花色素-1是早期慢性淋巴细胞白血病疾病进展的一个有价值的预测指标，因此它可以凭借简化的定量方法这一优势引入临床实践。
