Damato Bertil, Dopierala Justyna, Klaasen Annelies, van Dijk Marcory, Sibbring Julie, Coupland Sarah E
Liverpool Ocular Oncology Centre, St Paul's Eye Clinic, Royal Liverpool University Hospital, Liverpool, United Kingdom.
Invest Ophthalmol Vis Sci. 2009 Jul;50(7):3048-55. doi: 10.1167/iovs.08-3165. Epub 2009 Jan 31.
To evaluate multiplex ligation-dependent probe amplification (MLPA) of uveal melanoma as a predictive tool for metastatic death.
Uveal melanoma specimens of 73 patients treated between 1998 and 2000 were included. DNA samples were analyzed with MLPA evaluating 31 loci on chromosomes 1, 3, 6 and 8, and the results were correlated with metastatic death.
The patients (27 women; 46 men) had a median age of 60.6 years and a median follow-up of 6.2 years. Metastatic death occurred in 28 patients, correlating most strongly with chromosome 3 losses and gains on 8q (Cox univariate analysis, P < 0.001). Chromosome 6, region p25, gains correlated with good survival (Cox univariate analysis, P = 0.003). Prediction of metastatic death was improved by considering equivocal chromosome 3 losses as abnormal and by taking account of multiple risk factors, such as 8q gains, tumor diameter, and histologic features indicative of high-grade malignancy.
MLPA analysis of uveal melanoma predicts metastatic death if statistically insignificant losses of chromosome 3 are considered together with gains in 8q as well as clinical stage and histologic grade of malignancy.
评估葡萄膜黑色素瘤的多重连接依赖性探针扩增（MLPA）作为转移性死亡的预测工具。
纳入1998年至2000年间接受治疗的73例患者的葡萄膜黑色素瘤标本。用MLPA分析DNA样本，评估1、3、6和8号染色体上的31个位点，并将结果与转移性死亡相关联。
患者（27名女性；46名男性）的中位年龄为60.6岁，中位随访时间为6.2年。28例患者发生转移性死亡，与3号染色体缺失和8q增益相关性最强（Cox单因素分析，P<0.001）。6号染色体p25区域增益与良好生存相关（Cox单因素分析，P = 0.003）。将3号染色体模棱两可的缺失视为异常，并考虑多个风险因素，如8q增益、肿瘤直径和提示高级别恶性肿瘤的组织学特征，可改善转移性死亡的预测。
如果将3号染色体统计学上无显著意义的缺失与8q增益以及临床分期和恶性组织学分级一起考虑，葡萄膜黑色素瘤的MLPA分析可预测转移性死亡。
