Dong Nuo, Li Wei, Lin Hui, Wu Huping, Li Cheng, Chen Wensheng, Qin Wenjuan, Quyang Luowa, Wang Hua, Liu Zuguo
Eye Institute and affiliated Xiamen Eye Center of Xiamen University, Fujian, China.
Invest Ophthalmol Vis Sci. 2009 Jun;50(6):2710-5. doi: 10.1167/iovs.08-2905. Epub 2009 Jan 31.
To investigate the differentiation of conjunctival epithelium and tear film function in pingueculae.
Twelve patients (12 eyes) who underwent simple excision for pingueculae were enrolled in the study. Immunostaining for K14, K19, K10, MUC5AC, Pax6, P63, Ki67, and K16 was performed on the pinguecula epithelium and normal conjunctival epithelium. Schirmer I test results and tear film break-up time (TFBUT) were evaluated just before and 1 month after surgery.
Abnormal epidermal differentiation of pinguecula tissue, as evidenced by a decline in or absence of Pax6 expression, was accompanied by a decline in or absence of K19 keratin and MUC5AC, and an increase in K10 and K14 keratin. Furthermore, the pinguecula epithelium was actively proliferating, as evidenced by positive expression of Ki67, P63, and K16 keratin. The Schirmer I test results did not indicate any significant differences before and after surgery. However, the TFBUT was significantly prolonged 1 month after surgery compared with before surgery.
Pinguecula is a condition of abnormal epithelial differentiation. It is characterized by squamous proliferation and metaplasia, resulting in instability of tear film with normal basic tear secretion.
研究睑裂斑结膜上皮的分化及泪膜功能。
12例（12眼）因睑裂斑行单纯切除术的患者纳入本研究。对睑裂斑上皮和正常结膜上皮进行K14、K19、K10、MUC5AC、Pax6、P63、Ki67和K16的免疫染色。在手术前及手术后1个月评估泪液分泌试验（Schirmer I试验）结果和泪膜破裂时间（TFBUT）。
睑裂斑组织出现异常的表皮分化，表现为Pax6表达下降或缺失，同时伴有K19角蛋白和MUC5AC减少，以及K10和K14角蛋白增加。此外，睑裂斑上皮呈活跃增殖，表现为Ki67、P63和K16角蛋白表达阳性。泪液分泌试验（Schirmer I试验）结果在手术前后无显著差异。然而，与手术前相比，手术后1个月泪膜破裂时间显著延长。
睑裂斑是一种上皮分化异常的疾病。其特征为鳞状上皮增生和化生，导致泪膜不稳定，但基础泪液分泌正常。
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