Redden P R, Huang Y S
Efamol Research Institute, Kentville, Nova Scotia, Canada.
J Chromatogr. 1991 Jun 14;567(1):21-7. doi: 10.1016/0378-4347(91)80305-v.
This report describes an improved separation and quantitation of lipid fractions in a total lipid extract by high-performance liquid chromatography using a modified solvent and gradient system delivered by dual pumps and incorporating a mass detector and autosampler. The detector responses for various lipid fractions (cholesteryl esters, triacylglycerols, free cholesterol, and seven major phospholipid classes) were fitted to a quadratic equation, y = ax2 + bx + c, and quantified after detector calibration by a computer. This new system has the advantage of automation and reproducible separation. The present method was applied to rat liver analysis.
本报告描述了一种通过高效液相色谱法对总脂质提取物中的脂质组分进行改进的分离和定量方法。该方法使用经改良的溶剂和梯度系统，由双泵输送，并配备质量检测器和自动进样器。对各种脂质组分（胆固醇酯、三酰甘油、游离胆固醇和七种主要磷脂类）的检测器响应拟合为二次方程y = ax² + bx + c，并在通过计算机进行检测器校准后进行定量。这种新系统具有自动化和可重复分离的优点。本方法已应用于大鼠肝脏分析。
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