Lee Sichul, An Gynheung
Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea.
Plant Cell Environ. 2009 Apr;32(4):408-16. doi: 10.1111/j.1365-3040.2009.01935.x. Epub 2009 Jan 14.
Uptake and translocation of micronutrients are essential for plant growth. These micronutrients are also important food components. We generated transgenic rice plants over-expressing OsIRT1 to evaluate its functional roles in metal homeostasis. Those plants showed enhanced tolerance to iron deficiency at the seedling stage. In paddy fields, this over-expression caused plant architecture to be altered. In addition, those plants were sensitive to excess Zn and Cd, indicating that OsIRT1 also transports those metals. As expected, iron and zinc contents were elevated in the shoots, roots and mature seeds of over-expressing plants. This demonstrates that OsIRT1 can be used for enhancing micronutrient levels in rice grains.
微量营养素的吸收和转运对植物生长至关重要。这些微量营养素也是重要的食物成分。我们培育了过表达OsIRT1的转基因水稻植株，以评估其在金属稳态中的功能作用。这些植株在幼苗期对缺铁表现出更强的耐受性。在稻田中，这种过表达导致植株形态发生改变。此外，这些植株对过量的锌和镉敏感，表明OsIRT1也运输这些金属。正如预期的那样，过表达植株的地上部、根部和成熟种子中的铁和锌含量升高。这表明OsIRT1可用于提高水稻籽粒中的微量营养素水平。
