Gersbach M, Boiko D L, Niclass C, Petersen C C H, Charbon E
Ecole Polytechnique Fédérale de Lausanne, Quantum Architecture Group, Lausanne, Switzerland.
Opt Lett. 2009 Feb 1;34(3):362-4. doi: 10.1364/ol.34.000362.
The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.
在双光子激发模式下，以前所未有的时间分辨率分析了高亲和力非比率型Ca2+指示剂俄勒冈绿BAPTA-1（OGB-1）的荧光衰减。结果表明，三重指数衰减最能拟合OGB-1的荧光动力学。我们提供了一个模型，用于精确测量非比率型钙指示剂的游离Ca2+浓度和解离常数。
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