适配体选择表达：一种用于快速单步选择和检测适配体的新方法。
Aptamer selection express: a novel method for rapid single-step selection and sensing of aptamers.
作者信息
Fan Maomian, McBurnett Shelly Roper, Andrews Carrie J, Allman Amity M, Bruno John G, Kiel Johnathan L
机构信息
Air Force Research Laboratories, RHPC, Brooks City-Base, TX, USA.
出版信息
J Biomol Tech. 2008 Dec;19(5):311-9.
Here we describe a new DNA capture element (DCE) sensing system, based on the quenching and dequenching of a double-stranded aptamer. This system shows very good sensitivity and thermal stability. While quenching, dequenching, and separating the DCE systems made from different aptamers (all selected by SELEX), an alternative method to rapidly select aptamers was developed-the Aptamer Selection Express (ASExp). This process has been used to select aptamers against different types of targets (Bacillus anthracis spores, Bacillus thuringiensis spores, MS-2 bacteriophage, ovalbumin, and botulinum neurotoxin). The DCE systems made from botulinum neurotoxin aptamers selected by ASExp have been investigated. The results of this investigation indicate that ASExp can be used to rapidly select aptamers for the DCE sensing system.
在此，我们描述了一种基于双链适体淬灭和去淬灭的新型DNA捕获元件（DCE）传感系统。该系统表现出非常好的灵敏度和热稳定性。在对由不同适体（均通过指数富集的配体系统进化技术筛选）制成的DCE系统进行淬灭、去淬灭和分离时，开发了一种快速筛选适体的替代方法——适体快速筛选法（ASExp）。该方法已用于筛选针对不同类型靶标的适体（炭疽芽孢杆菌孢子、苏云金芽孢杆菌孢子、MS-2噬菌体、卵清蛋白和肉毒杆菌神经毒素）。对通过ASExp筛选的肉毒杆菌神经毒素适体制成的DCE系统进行了研究。该研究结果表明，ASExp可用于为DCE传感系统快速筛选适体。
Zotero 插件