Hamula Camille L A, Zhang Hongquan, Li Feng, Wang Zhixin, Chris Le X, Li Xing-Fang
Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, 10-102 Clinical Sciences Bldg., University of Alberta, Edmonton, Alberta, Canada T6G 2G3.
Trends Analyt Chem. 2011 Nov;30(10):1587-1597. doi: 10.1016/j.trac.2011.08.006. Epub 2011 Sep 9.
DNA aptamers specifically recognizing microbial cells and viruses have a range of analytical and therapeutic applications. This article describes recent advances in the development of aptamers targeting specific pathogens (e.g., live bacteria, whole viral particles, and virally-infected mammalian cells). Specific aptamers against pathogens have been used as affinity reagents to develop sandwich assays, to label and to image cells, to bind with cells for flow-cytometry analysis, and to act as probes for development of whole-cell biosensors. Future applications of aptamers to pathogens will benefit from recent advances in improved selection and new aptamers containing modified nucleotides, particularly slow off-rate modified aptamers (SOMAmers).
特异性识别微生物细胞和病毒的DNA适配体具有一系列分析和治疗应用。本文描述了靶向特定病原体（如活细菌、完整病毒颗粒和病毒感染的哺乳动物细胞）的适配体开发的最新进展。针对病原体的特异性适配体已被用作亲和试剂，以开发夹心测定法、标记和成像细胞、与细胞结合用于流式细胞术分析，以及用作开发全细胞生物传感器的探针。适配体在病原体方面的未来应用将受益于改进筛选和含修饰核苷酸的新适配体（特别是解离速率慢的修饰适配体，即SOMAmers）的最新进展。
