University of Warmia and Mazury, Faculty of Environmental Protection and Fisheries, Olsztyn-Kortowo, Poland.
Comp Biochem Physiol A Mol Integr Physiol. 2009 Jun;153(2):113-9. doi: 10.1016/j.cbpa.2009.01.003. Epub 2009 Jan 13.
Studies on larval rearing of silver bream (Vimba vimba), a migratory cyprinid fish have addressed on limited scale larval and juvenile rearing using commercial and semipurified diets along with live feeds, such as brine shrimp Artemia nauplii. The objectives of the present study were (1) to determine whether experimental, protein-, peptide-, free amino acid-based diets are adequate for larval silver bream, a stomachless fish, (2) to evaluate whether commercial and purified diets are comparable as the first/exclusive feed for growth and survival of silver bream, and (3) to examine whether free amino acid concentrations in fish body are potential indicators of availability of amino acid sources. We report here the differences in diets acceptance, fish growth and diet utilization in silver bream in comparison to other cyprinid fishes. We specifically address the response in free amino acids in the body to dietary treatments. Experimental diets included: a commercial Aglo Norse feed, casein-gelatin based diet (CG), free amino acid mixture diet (FAA), dipeptide (PP), dipeptide-protein (PP50) based diet, and dipeptide diet without arginine (NoArg). In addition, live Artemia were offered to 3 groups and "fasting" control treatment was included during 3 week long trial. Fish offered Artemia overperformed those offered formulated diets both in terms of mass (80.7+/-26.3 mg) and survival (97.2%). We also indicate that commercial and purified diets are comparable as the first/exclusive feed for growth and survival of silver bream. Our experiment also showed that the whole body free amino acid concentrations of 9 indispensable amino acids (IDAA) out of 10 (His, Thr, Arg, Val, Met, Ile, Leu, Trp, Lys) in the PP50 group was the highest among 7 diet treatments and the totalfree amino acid concentration, total dispensable amino acids (DAA) and total IDAA of the PP50 diet fed fish showed the same trend. This may indicate that diets based on 50% of dipeptides and 50% of protein are adequate for silver bream larvae as they result in similar growth, survival and whole body IDAA concentrations as in fish fed CG (protein-based) diet fed fish.
关于银鲴（Vimba vimba）幼虫饲养的研究，是在有限的规模上，使用商业的和半纯化的饲料以及活饲料，如卤虫无节幼体，对仔鱼和幼鱼进行的。本研究的目的是：（1）确定实验性的、基于蛋白质、肽、游离氨基酸的饲料是否适合无胃鱼类银鲴的幼鱼；（2）评估商业和纯化的饲料是否可以作为银鲴生长和存活的首选/唯一饲料；（3）研究鱼体中的游离氨基酸浓度是否是氨基酸来源可用性的潜在指标。在此，我们报告了银鲴在饲料接受度、生长和饲料利用率方面与其他鲤科鱼类的差异。我们特别关注了对不同饲料处理的游离氨基酸的反应。实验性饲料包括：一种商业的 Aglo Norse 饲料、酪蛋白-明胶基础饲料（CG）、游离氨基酸混合物饲料（FAA）、二肽（PP）、二肽-蛋白质（PP50）基础饲料和不含精氨酸的二肽饲料（NoArg）。此外，还向 3 个组提供活卤虫，并在 3 周的试验期间包括“禁食”对照组。提供活卤虫的鱼在质量（80.7+/-26.3mg）和存活率（97.2%）方面都优于提供配方饲料的鱼。我们还表明，商业和纯化的饲料在作为银鲴生长和存活的首选/唯一饲料方面是可以相互替代的。我们的实验还表明，在 7 种饲料处理中，PP50 组的 9 种必需氨基酸（IDAA）中的 9 种游离氨基酸（His、Thr、Arg、Val、Met、Ile、Leu、Trp、Lys）的全身体内游离氨基酸浓度最高，而 PP50 饲料喂养的鱼的总游离氨基酸浓度、总可利用氨基酸（DAA）和总 IDAA 也呈现出相同的趋势。这可能表明，基于 50%二肽和 50%蛋白质的饲料对银鲴幼鱼是足够的，因为它们在生长、存活率和全身体内 IDAA 浓度方面与喂养 CG（基于蛋白质）饲料的鱼相似。
