Prostak K S, Seifert P, Skobe Z
Forsyth Dental Center, Department of Electron Microscopy, Boston, Massachusetts 02115.
J Dent Res. 1991 Sep;70(9):1266-71. doi: 10.1177/00220345910700090701.
We investigated diverse groups of fish species to determine whether the fluorine (F) contents of the dental hard tissues were related to baseline serum F levels. Serum samples, enameloid, dentin, ganoid/enamel, and bone were analyzed for F by either electron microprobe or wet chemistry. Species were categorized into two groups based on the F content of the enameloid. One group contained greater than 2.6 wt% F in enameloid, whereas the other group had less than 0.45 wt% F in enameloid. The dentin and bone from all species (or, in skates, the cartilage), as well as the ganoid/enamel layer of a Holostean fish (alligator gar), showed consistently low F content. In those species whose teeth developed in sequential rows, the F content of enameloid increased with progressive tooth development. The serum F levels of all fish were below 0.05 microgram F/mL (2.63 mumol/L) and were not significantly related to the F content of the enameloid. The results substantiate the idea that F incorporation into enameloid is related to fish phylogeny, not food or habitat. It is suggested that specialized outer dental epithelial cell configurations may facilitate the incorporation of F into enameloid.
我们研究了不同种类的鱼类，以确定牙齿硬组织中的氟（F）含量是否与血清F水平基线相关。通过电子微探针或湿化学分析法对血清样本、釉质、牙本质、硬鳞/釉质和骨骼中的F进行了分析。根据釉质中的F含量，将鱼类分为两组。一组的釉质中F含量大于2.6 wt%，而另一组的釉质中F含量小于0.45 wt%。所有物种的牙本质和骨骼（或鳐鱼的软骨），以及全骨鱼类（雀鳝）的硬鳞/釉质层，F含量始终较低。在那些牙齿呈连续排生长的物种中，釉质中的F含量随着牙齿的逐渐发育而增加。所有鱼类的血清F水平均低于0.05微克F/毫升（2.63微摩尔/升），且与釉质中的F含量无显著相关性。结果证实了F掺入釉质与鱼类系统发育有关，而非与食物或栖息地有关的观点。有人提出，特殊的牙齿外上皮细胞结构可能有助于F掺入釉质。
