Allouche Lynda, Hamadouche Mohamed, Touabti Abderrezek
Department of Biology, Faculty of Sciences, University of Setif, Algeria.
Exp Toxicol Pathol. 2009 Sep;61(5):503-10. doi: 10.1016/j.etp.2008.12.003. Epub 2009 Feb 1.
The current study evaluates the impact of low or moderate levels of lead acetate (PbAc) on sperm parameters, gonadotropins (FSH, LH) and testosterone. Adult albino wistar male rats were allocated to five groups and given 0%, 0.025%, 0.05%, 0.1% and 0.3% PbAc in distilled drinking water for 24 weeks. There was no change in body weight gain and in absolute or relative weight of testes, epididymides and seminal vesicles. The ventral prostate weight was decreased in groups exposed to 0.05%, 0.1% and 0.3% PbAc without statistically significant differences. Sperm velocity was decreased in all treated groups while reduction of sperm motility was observed in rats exposed to 0.05%, 0.1% and 0.3% PbAc without statistically significant differences compared to the control group. However, there was a significant increase greater than 100% in the total percentage of abnormal sperm in groups treated with 0.1% (p<0.01) and 0.3% PbAc (p<0.05).The frequency of dead sperm was significantly increased only in the 0.3% PbAc group (p<0.01). Significant increases in frequencies of amorphous head sperm (p<0.01) and abnormal tails (p<0.01) were found in the group exposed to 0.1% PbAc, while the frequency of neck abnormalities was increased in the high-lead-exposed group (p<0.01). The levels of LH and FSH were not significantly affected after lead treatment and significant increase in serum testosterone level was noted only in animals administered 0.05% PbAc (p<0.01). In conclusion, our results reveal that some sperm parameters were altered at low or moderate lead concentrations with no obvious alteration of hypothalamic-pituitary function. The increase of testosterone level observed suggests that lead may target testicular function.
本研究评估了低水平或中等水平醋酸铅（PbAc）对精子参数、促性腺激素（FSH、LH）和睾酮的影响。将成年白化Wistar雄性大鼠分为五组，分别给予含0%、0.025%、0.05%、0.1%和0.3% PbAc的蒸馏水，持续24周。体重增加以及睾丸、附睾和精囊的绝对重量或相对重量均无变化。暴露于0.05%、0.1%和0.3% PbAc的组中，腹侧前列腺重量有所下降，但无统计学显著差异。所有处理组的精子速度均降低，而在暴露于0.05%、0.1%和0.3% PbAc的大鼠中观察到精子活力降低，与对照组相比无统计学显著差异。然而，在接受0.1%（p<0.01）和0.3% PbAc（p<0.05）处理的组中，异常精子的总百分比显著增加超过100%。仅在0.3% PbAc组中，死精频率显著增加（p<0.01）。在暴露于0.1% PbAc的组中，发现无定形头部精子频率（p<0.01）和异常尾部频率（p<0.01）显著增加，而在高铅暴露组中，颈部异常频率增加（p<0.01）。铅处理后，LH和FSH水平未受到显著影响，仅在给予0.05% PbAc的动物中观察到血清睾酮水平显著升高（p<0.01）。总之，我们的结果表明，在低铅或中等铅浓度下，一些精子参数发生了改变，而下丘脑-垂体功能无明显改变。观察到的睾酮水平升高表明铅可能作用于睾丸功能。
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