Ma Xiaoying, Cheng Xuemin, Li Furan, Guo Junsheng
Department of Environmental Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Wei Sheng Yan Jiu. 2008 Nov;37(6):733-5.
To study the endocrine disturbing effect of fluorin on Hypothalamus-Hypophysis-Testis axis in male rats.
A total of 36 Wister male rats weighting 60-70 g were randomly divided into group I (high fluoride group of F-100 mg/l), group II (low fluoride group of F- 30 mg/l), group III (control group with pure water), with 12 rats in each group. Fluoride was administered with drinking water for 8 weeks. Then the level of procreation hormone in serum was detected by RIA method. And the spermatozoa quality was analysized.
There was difference between group I, group II and group III each other (P < 0.05) in body weight. As to right testis weight, there was difference between group I, group II and group III each other (P < 0.05). Epididymide organic coefficient in group II and group I were lower than that in group III (P > 0.05). Compared with group III, the counts amount of sperm and the rates of sperm mobility in group II and group I singnificantly increased (P < 0.05), and the rates of sperm aberration in group II and group I significantly decreased (P < 0.05), compared with group II, the sperm quality of group I descreased significantly (P < 0.05). The level of GnRH in three groups were significant difference between each groups (P <0.05). The level of FSH in three groups were significant difference between each groups (P < 0.05). The level of ICSH in three groups were no significant difference between each groups (P > 0.05). The level of T in Group I is significant lower than that of in Group II and Group III (P < 0.05). The level of E2 in Group I is significant higher than that of in Group II and Group III (P < 0.05).
These data support our hypothesis that fluorin have no significant effect on the function of male Rat Hypothalamus, that fluorin may effect the function of male Rat Hypophysis, that T/ICSH can be used to find the functional change of Leydig cells. Fluorin could affect hormone levels of each layer of the Hypothalamus-Hypophysis-Testis axis, and show the male reproductive endocrine disturbing effects.
研究氟对雄性大鼠下丘脑-垂体-睾丸轴的内分泌干扰作用。
将36只体重60 - 70 g的Wister雄性大鼠随机分为I组（高氟组，氟含量为100 mg/l）、II组（低氟组，氟含量为30 mg/l）、III组（纯水对照组），每组12只。通过饮用水给予氟8周。然后采用放射免疫分析法检测血清中生殖激素水平，并分析精子质量。
I组、II组和III组在体重方面相互之间存在差异（P < 0.05）。右侧睾丸重量方面，I组、II组和III组相互之间存在差异（P < 0.05）。II组和I组的附睾脏器系数低于III组（P > 0.05）。与III组相比，II组和I组的精子计数和精子活动率显著升高（P < 0.05），II组和I组的精子畸变率显著降低（P < 0.05），与II组相比，I组的精子质量显著下降（P < 0.05）。三组的GnRH水平在各组之间存在显著差异（P < 0.05）。三组的FSH水平在各组之间存在显著差异（P < 0.05）。三组的ICSH水平在各组之间无显著差异（P > 0.05）。I组的T水平显著低于II组和III组（P < 0.05）。I组的E2水平显著高于II组和III组（P < 0.05）。
这些数据支持我们的假设，即氟对雄性大鼠下丘脑功能无显著影响，氟可能影响雄性大鼠垂体功能，T/ICSH可用于发现睾丸间质细胞的功能变化。氟可影响下丘脑-垂体-睾丸轴各层的激素水平，并表现出对雄性生殖内分泌的干扰作用。
