Department of Orthopaedic Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
J Biomed Mater Res A. 2010 Jan;92(1):297-302. doi: 10.1002/jbm.a.32356.
Exogenous crosslinking was proved to improve the fatigue resistance of anulus fibrosus and the stability of motion segment. The effect of crosslinking on the recovery of stab-injured discs, however, was less studied. The purpose of this study is to find if the exogenous crosslinking can increase the mechanical function of injured discs. Fresh healthy porcine discs (T2/T9) from 6-month-old swine were obtained immediately following death. Anular puncture using 16 and 18 G spinal needle were used to create medium and large disc stab injury models. Three treatments were designed for each injury model. The first one is the injured discs without treatment. The second one is the injured discs soaked with phosphate buffered solution for 2 days. The third one is the injured discs soaked with 0.33% genipin solution for 2 days. The disc integrity was evaluated using quantitative discomanometry (QD) apparatus. Four QD parameters, that is, the leakage pressure and volume, and the saturate pressure and volume, were analyzed to find the efficacy of treatment. We found that soaking of genipin solution recovered the disc leakage pressure from 1.3 to 1.8 MPa in 16 G-injury-model and from 2.3 to 3.2 MPa in 18 G-injury-model, and recovered the saturate pressure from 1.6 to 2.0 MPa in 16 G-injury-model and from 2.7 to 3.7 MPa in 18 G-injury-model. The improvement of disc integrity by soaking with genipin solution indicate that the exogenous crosslinking may help the biomechanical performance of an injured disc.
外源性交联被证明可以提高纤维环的抗疲劳性和运动节段的稳定性。然而，交联对刺伤椎间盘的恢复效果的研究较少。本研究的目的是探讨外源性交联是否可以增加受伤椎间盘的力学功能。从 6 月龄猪中立即获得新鲜健康的猪椎间盘（T2/T9）。使用 16 和 18 G 脊柱针进行环形穿刺，以创建中等和大椎间盘刺伤模型。为每个损伤模型设计了三种治疗方法。第一种是未经处理的受伤椎间盘。第二种是用磷酸盐缓冲溶液浸泡 2 天的受伤椎间盘。第三种是用 0.33%京尼平溶液浸泡 2 天的受伤椎间盘。使用定量椎间盘仪（QD）评估椎间盘完整性。分析了四个 QD 参数，即泄漏压力和体积，以及饱和压力和体积，以找到治疗效果。我们发现，京尼平溶液浸泡可使 16 G 损伤模型中的椎间盘泄漏压力从 1.3 MPa 恢复到 1.8 MPa，使 18 G 损伤模型中的椎间盘泄漏压力从 2.3 MPa 恢复到 3.2 MPa；使 16 G 损伤模型中的椎间盘饱和压力从 1.6 MPa 恢复到 2.0 MPa，使 18 G 损伤模型中的椎间盘饱和压力从 2.7 MPa 恢复到 3.7 MPa。京尼平溶液浸泡可提高椎间盘完整性，表明外源性交联可能有助于受伤椎间盘的生物力学性能。
