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[在牛生长激素存在下体外培养的鸡胚胫骨原基的组织化学和组织酶学研究]

[Histochemical and histoenzymatic studies of chick-embryo tibial rudiments cultured in vitro in the presence of bovine growth hormones].

作者信息

Renda T, D'Este L, Bronzetti E

出版信息

Riv Istochim Norm Patol. 1976;20(4):197-207.

PMID:191896
Abstract

The AA. have studied the effect of the bovine GH on the cellular and intercellular components of the chick embryo tibias cultured organotypically. They have observed a strong increase of the proliferative activity of the chondroblasts such as showed by a stronger uptake of the tritiated timidine by traited tibias in comparison with the control tibias. The chondroblasts of the traited tibias show an increase of some enzymatic activities connected with the aerobic cycle and of the UDPG-DH, connected with the uronic acids cycle. In the amorphous matrix of the traited tibias they have showed a greater polimerisation and sulfation of the condroitinesulfate A and C, which, almost exclusively, constitute the mucopolisaccharidic component of the matrix.

摘要

研究人员研究了牛生长激素对以器官型方式培养的鸡胚胫骨的细胞和细胞间成分的影响。他们观察到，与对照胫骨相比，经处理的胫骨对氚标记胸腺嘧啶核苷的摄取更强，这表明软骨细胞的增殖活性显著增加。经处理的胫骨的软骨细胞显示出与有氧循环相关的一些酶活性以及与糖醛酸循环相关的UDPG-DH有所增加。在经处理的胫骨的无定形基质中，他们发现硫酸软骨素A和C的聚合和硫酸化程度更高，而硫酸软骨素A和C几乎是基质中粘多糖成分的唯一构成部分。

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