Hasaniya Nahidh W, Premaratne Shyamal, Zhang Wayne W, Razzuk Aziz M, Abdul-Ghani Ayman A, Siera Milea, Dashwood Roderick H, Eklof Bo, Tinsley Larry R, McNamara J Judson
Department of Surgery, School of Medicine, Loma Linda University, Loma Linda, California, USA.
Vasc Endovascular Surg. 2009 Apr-May;43(2):170-7. doi: 10.1177/1538574408328585. Epub 2009 Feb 3.
The primary objectives of this study were to determine the time course of ischemia-reperfusion injury in an isolated rabbit lung model and to quantify this damage using electron microscopic methodology coupled with statistical analyses.
Eight groups of isolated rabbit lungs (n = 5 per group) were subjected to predetermined periods of ischemia-reperfusion. Two hours of ischemia and 4 hours of reperfusion were concluded to be necessary to induce optimal ischemia-reperfusion injury in this model. Four other groups were subjected to 2 hours of ischemia followed by selected periods of reperfusion. These groups were compared to 4 control groups that were perfused for comparable time periods but without the initial ischemia. New quantitative methods were developed based on the average surface area of the alveoli and average number of alveoli per unit surface area, using scanning electron microscopic examination.
Ischemia per se caused substantial damage. Restoration of volume and nutrients reversed this damage at 1 hour of reperfusion, but severe damage was evident at 4 hours of reperfusion, as reported by subjective and blinded examination. By using the new quantitative methods, there was a significant difference between the groups (P < .005) according to the time of post-ischemia-reperfusion, which correlated with the subjective evaluation of damage.
These 2 new quantitative techniques provide an objective assessment of damage in the isolated rabbit lung model, suggesting that they warrant further consideration in similar studies of ischemia reperfusion injury.
本研究的主要目的是确定离体兔肺模型中缺血再灌注损伤的时间进程，并使用电子显微镜方法结合统计分析对这种损伤进行量化。
八组离体兔肺（每组n = 5）经历预定的缺血再灌注时间。在该模型中，得出2小时缺血和4小时再灌注是诱导最佳缺血再灌注损伤所必需的。另外四组经历2小时缺血，随后进行选定时间段的再灌注。将这些组与4个对照组进行比较，对照组在相当的时间段内进行灌注，但无初始缺血。基于肺泡的平均表面积和每单位表面积的肺泡平均数量，利用扫描电子显微镜检查开发了新的定量方法。
缺血本身造成了严重损伤。主观和盲法检查表明，在再灌注1小时时，容积和营养物质的恢复逆转了这种损伤，但在再灌注4小时时严重损伤明显。通过使用新的定量方法，根据缺血再灌注后的时间，各组之间存在显著差异（P <.005），这与损伤的主观评估相关。
这两种新的定量技术为离体兔肺模型中的损伤提供了客观评估，表明它们在类似的缺血再灌注损伤研究中值得进一步考虑。
