Choi Jin Hee, Kim Yangseon, Lee Yong-Hwan
Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Center for Fungal Genetic Resources, Seoul National University, Seoul, Korea.
J Microbiol Biotechnol. 2009 Jan;19(1):11-6.
Magnaporthe oryzae, the causal agent of rice blast, forms a specialized infection structure, called an appressorium, which is crucial for penetration and infection of the host plant. Pharmacological data suggest that calcium/calmodulin-dependent signaling is involved in appressorium formation in this fungus. To understand the role of the calcium/ calmodulin-activated protein phosphatase on appressorium formation at the molecular level, MCNA, a gene encoding the catalytic subunit of calcineurin, was functionally characterized in M. oryzae. Transformants expressing sense/antisense RNA of MCNA exhibited significant reductions in mycelial growth, conidiation, appressorium formation, and pathogenicity. cDNA of MCNA functionally complemented a calcineurin disruptant strain (cmp1::LEU2 cmp2::HIS3) of Saccharomyces cerevisiae. These data suggest that calcineurin A plays important roles in signal transduction pathways involved in the infection-related morphogenesis and pathogenicity of M. oryzae.
稻瘟病菌是水稻稻瘟病的致病因子，它会形成一种特殊的侵染结构，称为附着胞，这对于侵染和感染寄主植物至关重要。药理学数据表明，钙/钙调蛋白依赖性信号传导参与了这种真菌中附着胞的形成。为了在分子水平上了解钙/钙调蛋白激活的蛋白磷酸酶在附着胞形成中的作用，对稻瘟病菌中编码钙调神经磷酸酶催化亚基的基因MCNA进行了功能表征。表达MCNA正义/反义RNA的转化体在菌丝生长、分生孢子形成、附着胞形成和致病性方面均显著降低。MCNA的cDNA在功能上互补了酿酒酵母的钙调神经磷酸酶缺陷菌株（cmp1::LEU2 cmp2::HIS3）。这些数据表明，钙调神经磷酸酶A在稻瘟病菌与感染相关的形态发生和致病性的信号转导途径中发挥重要作用。
