孤啡肽的中枢给药通过自由活动的去卵巢大鼠正中隆起处的ORL1受体抑制促性腺激素释放激素的分泌。
Central administration of Orphanin FQ inhibits GnRH secretion by ORL1 receptor in the median eminence of freely moving ovariectomized rats.
作者信息
An Xiao-Fei, He Ming, Feng Yi, Feng Hao, Yu Jiang-Yi
机构信息
Department of Endocrinology, Jiangsu Province Hospital of Traditional Chinese Med, Nanjing 210029, China.
出版信息
Neurosci Bull. 2009 Feb;25(1):1-6. doi: 10.1007/s12264-009-1119-5.
OBJECTIVE
This study aimed to investigate the possible role of Orphanin FQ (OFQ) in the regulation of hypo-thalamic gonadotropin-releasing hormone (GnRH) secretion.
METHODS
The method of push-pull perfusion and radioimmuno-assay (RIA) were adopted to examine the secretory profile of GnRH in the median eminence (ME) in freely moving ovari-ectomized (OVX) rats after intracerebroventricular (icv) injection of OFQ and/or [Nphe(1)]NC(1-13)NH(2) (NC13), a competitive antagonists of the opioid receptor-like 1 receptor (ORL1 receptor).
RESULTS
GnRH release from ME significantly decreased from 40 min to 80 min after the administration of 20 and 200 nmol OFQ in OVX rats (P < 0.05). This inhibitory effect of 20 nmol OFQ could be abolished by pretreatment with equal dose of NC13. More interestingly, GnRH secretion from ME was increased markedly 60 min after icv injection of 100 and 200 nmol NC13 (P < 0.05).
CONCLUSION
Our results suggested central administration of OFQ could inhibit the release of GnRH in the ME of hypothalamus through ORL1 receptor, providing further in vivo evidence supporting the role of OFQ in the control of GnRH secretion.
目的
本研究旨在探讨孤啡肽（OFQ）在下丘脑促性腺激素释放激素（GnRH）分泌调节中的可能作用。
方法
采用推挽式灌注法和放射免疫分析法（RIA），检测脑室注射OFQ和/或[Nphe(1)]NC(1-13)NH(2)（NC13，阿片样物质受体-1（ORL1受体）的竞争性拮抗剂）后，自由活动的去卵巢（OVX）大鼠正中隆起（ME）中GnRH的分泌情况。
结果
在OVX大鼠中，注射20和200 nmol OFQ后40至80分钟，ME中GnRH的释放显著减少（P<0.05）。等量的NC13预处理可消除20 nmol OFQ的这种抑制作用。更有趣的是，脑室注射100和200 nmol NC13 60分钟后，ME中GnRH的分泌显著增加（P<0.05）。
结论
我们的结果表明，中枢给予OFQ可通过ORL1受体抑制下丘脑ME中GnRH的释放，为OFQ在GnRH分泌控制中的作用提供了进一步的体内证据。
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