高浓度的钴胺素传递蛋白会干扰人体血清和乳汁中钴胺素的常规检测。一个问题及其解决方案。

High concentrations of haptocorrin interfere with routine measurement of cobalamins in human serum and milk. A problem and its solution.

作者信息

Lildballe Dorte L, Hardlei Tore F, Allen Lindsay H, Nexo Ebba

机构信息

Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.

出版信息

Clin Chem Lab Med. 2009;47(2):182-7. doi: 10.1515/CCLM.2009.047.

DOI:10.1515/CCLM.2009.047

PMID:19191724

Abstract

BACKGROUND

Human milk and occasional serum samples contain high concentrations of unsaturated haptocorrin, which influence accurate measurement of cobalamins.

METHODS

Cobalamins in serum samples spiked with increasing amounts of unsaturated haptocorrin were measured employing the Centaur, Cobas and Architect analysers. Cobinamide-coated EAH sepharose was employed for pretreatment of the samples. Human milk samples were collected from 24 healthy mothers. Haptocorrin was measured by ELISA.

RESULTS

The measured concentration of cobalamins either increased (Centaur analyser) or decreased (Architect, Cobas analysers) significantly for haptocorrin >10 nM, and was 220%, 52% or 45% of the expected values in a serum sample containing 50 nM haptocorrin. Following pretreatment with cobinamide-sepharose, the expected cobalamin concentration was obtained (Centaur). The milk samples contained 4.5-180 nM haptocorrin. In samples containing >10 nM haptocorrin (n=19), the median concentration of cobalamins decreased from 1.3 nM to 0.67 nM after pretreatment with cobinamide-sepharose.

CONCLUSIONS

Haptocorrin in concentrations above 10 nM influences measurement of cobalamins giving rise to falsely elevated or decreased results. Removal of unsaturated haptocorrin by pretreatment with cobinamide-sepharose solves the problem.

摘要

背景

人乳和偶尔的血清样本中含有高浓度的不饱和钴胺素传递蛋白，这会影响钴胺素的准确测量。

方法

使用Centaur、Cobas和Architect分析仪测量添加了不同量不饱和钴胺素传递蛋白的血清样本中的钴胺素。采用钴胺酰胺包被的环氧氯丙烷活化琼脂糖（EAH sepharose）对样本进行预处理。从24名健康母亲处采集人乳样本。通过酶联免疫吸附测定（ELISA）测量钴胺素传递蛋白。

结果

当钴胺素传递蛋白>10 nM时，测量的钴胺素浓度显著升高（Centaur分析仪）或降低（Architect、Cobas分析仪），在含有50 nM钴胺素传递蛋白的血清样本中，测量值分别为预期值的220%、52%或45%。用钴胺酰胺琼脂糖预处理后，获得了预期的钴胺素浓度（Centaur）。人乳样本中含有4.5 - 180 nM的钴胺素传递蛋白。在含有>10 nM钴胺素传递蛋白的样本（n = 19）中，用钴胺酰胺琼脂糖预处理后，钴胺素的中位浓度从1.3 nM降至0.67 nM。