Wilson E M, Holcombe S J, Lamar A, Hauptman J G, Brooks M B
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
J Vet Intern Med. 2009 Mar-Apr;23(2):323-8. doi: 10.1111/j.1939-1676.2008.0254.x. Epub 2009 Feb 3.
The extent of preservation of clotting factors and incidence of transfusion reactions to noncommercial equine plasma is not documented.
Equine frozen plasma would retain its coagulation factor activity within the reference range and the incidence of transfusion reactions would be low.
Ten plasma donor horses. Fifty clinically ill hospitalized horses receiving plasma were reviewed to determine the incidence of reactions.
In vitro study and retrospective case review. Plasma was prepared by gravity sedimentation from whole blood refrigerated for 48 hours. The activities of factors VII through XII, antithrombin (AT), and Protein C were measured. Factor activities were compared for plasma samples obtained before blood collection (S0), after 48 hours of gravity sedimentation at 5 degrees C and after plasma separation (S1), and after 90 days of storage at -20 degrees C (S90). The medical records of 50 consecutive clinically ill horses receiving frozen plasma were reviewed to determine the incidence of transfusion reactions.
The combined effect of plasma harvest, gravity sedimentation, decantation, and freezing caused significant reductions in factors IX, (43%P= .0013), X, (33%P= .0001), XI, (48%P= .0008), AT, (10%P= .02), and Protein C (26%P= .0001). Activities for all factors analyzed, except factor X, remained within the reference ranges. Transfusion reactions were recorded for 5/50 horses.
Clotting factors, AT, and Protein C were well preserved. The incidence of reactions to frozen plasma was 10%.
关于非商业来源马血浆中凝血因子的保存程度及输血反应发生率尚无文献记载。
马冷冻血浆的凝血因子活性将维持在参考范围内，且输血反应发生率较低。
10匹血浆供体马。对50匹接受血浆治疗的临床患病住院马进行回顾性研究以确定反应发生率。
体外研究及回顾性病例分析。通过重力沉降法从冷藏48小时的全血中制备血浆。检测因子VII至XII、抗凝血酶（AT）及蛋白C的活性。比较采血前（S0）、5℃重力沉降48小时并分离血浆后（S1）以及-20℃储存90天后（S90）的血浆样本的因子活性。回顾50匹连续接受冷冻血浆治疗的临床患病马的病历，以确定输血反应发生率。
血浆采集、重力沉降、倾析及冷冻的综合作用导致因子IX（43%，P = 0.0013）、X（33%，P = 0.0001）、XI（48%，P = 0.0008）、AT（10%，P = 0.02）及蛋白C（26%，P = 0.0001）显著降低。除因子X外，所有分析因子的活性均保持在参考范围内。50匹马中有5匹记录到输血反应。
凝血因子、AT及蛋白C保存良好。冷冻血浆的反应发生率为10%。
