Sasamoto Minoru, Nagai Atsushi, Sakagami Ryuji, Kitamura Kenji, Miki Takeyoshi
Department of Periodontology, Fukuoka Dental College, Fukuoka, Japan.
J Int Acad Periodontol. 2009 Jan;11(1):138-46.
Nearly 400 species of bacteria have been found in human periodontal pockets, and half of them remain uncultured to date. The diagnosis of individual periodontal microflora using culture-independent detection of species is expected to serve as a tool for 'tailor-made' periodontal treatments. However, the richness and abundance of bacterial species within individual subgingival microflora have not been sufficiently studied to develop more specific diagnostic microbiological tests. The purpose of this study was to determine the ecological architecture of the subgingival microflora among chronic periodontitis patients and their individual differences based on phylotype analyses.
Four 16S rRNA gene libraries were constructed from subgingival plaque samples taken from all diseased sites in four chronic periodontitis patients. The 480 clones generated from each of the four libraries were analyzed by phylotyping and subjected to ecological estimation of species richness.
The indices of species richness of the four libraries were 73, 75, 98 or 100 phylotypes. A total of 195 phylotypes were identified in the combined libraries. The majority of phylotypes were assigned to the Bacteroidetes, Fusobacteria, Proteobacteria, Firmicutes and Actinobacteria phyla. The phylotypes assigned to the Bacteroidetes and Fusobacteria phyla were the most predominant in each library (chi2-test, p < 0.05).
In this study, the molecular ecology of 1920 clones obtained from four patients' subgingival plaque samples was examined. More than half of the detected clones were categorized under either the Bacteroidetes or the Fusobacteria phyla. Each library showed unique richness and abundance of phylotypes.
在人类牙周袋中已发现近400种细菌，其中一半至今仍未培养成功。利用不依赖培养的物种检测方法诊断个体牙周微生物群落有望成为“量身定制”牙周治疗的工具。然而，为了开发更具特异性的诊断微生物学检测方法，对个体龈下微生物群落中细菌物种的丰富度和丰度尚未进行充分研究。本研究的目的是基于系统发育型分析确定慢性牙周炎患者龈下微生物群落的生态结构及其个体差异。
从4例慢性牙周炎患者所有患病部位采集的龈下菌斑样本构建了4个16S rRNA基因文库。对4个文库中每个文库产生的480个克隆进行系统发育型分析，并对物种丰富度进行生态评估。
4个文库的物种丰富度指数分别为73、75、98或100个系统发育型。在合并文库中总共鉴定出195个系统发育型。大多数系统发育型被归为拟杆菌门、梭杆菌门、变形菌门、厚壁菌门和放线菌门。在每个文库中，归为拟杆菌门和梭杆菌门的系统发育型最为占优势（卡方检验，p < 0.05）。
本研究检测了从4例患者龈下菌斑样本中获得的1920个克隆的分子生态学。超过一半的检测克隆被归类于拟杆菌门或梭杆菌门。每个文库都显示出独特的系统发育型丰富度和丰度。
