嗅鞘细胞可以减少挫伤性脊髓组织的丢失，但不能减少脊髓空洞的形成。

Olfactory ensheathing cells can reduce the tissue loss but not the cavity formation in contused spinal cord of rats.

机构信息

Research Institute of Surgery, Daping Hospital ,Third Military Medical University, Chongqing 400042, China.

出版信息

J Neurol Sci. 2011 Apr 15;303(1-2):67-74. doi: 10.1016/j.jns.2011.01.013.

DOI:10.1016/j.jns.2011.01.013

Abstract

In recent years, olfactory ensheathing cells (OECs) have been used as a therapeutic strategy to repair the anatomical structure and promote the function recovery of injured spinal cord in both animal and human. In this study, OECs were transplanted into contused spinal cords of adult rats. After dorsal laminectomy at T10 vertebra, spinal cord was injured by a force of 10 g with NYU II impactor from 25 mm above the exposed cord. The contused spinal cord received injections of OECs in DMEM or DMEM alone at one week after injury. The migration and distribution of OECs in the contused spinal cord were observed by the light microscope. The intact tissue area, injured tissue area, cavity size, number of myelinated nerve fibers and neurons labeled by CB-HRP in T8 segment were measured and counted by the semi-quantitative techniques at 6 weeks after transplantation. Locomotor ability and conductive function of the spinal cord were evaluated by the BBB score and cortical somatosensory evoked potentials (CSEP) recording. OECs were found in both lesion site and tissue near the lesion. The intact tissue area was significantly larger in the OECs-transplanted rats than that in the DMEM-injected animals, whereas the injured tissue area was significantly smaller in the OECs-rats than that in the DMEM-rats. The number of myelinated nerve fibers in the lesion site and preserved neurons in T8 was significantly greater in the OECs-group than in the DMEM-group, but the cavity size detected was not significantly different between the two groups. The BBB score and CSEP recording showed a better performance of locomotor ability and conductive function in the OECs-transplanted rats than in the DMEM-injected animals. These results indicate that OECs can counteract secondary tissue degeneration after spinal cord injury. Although they cannot reduce the cavity formation, they can promote morphological preservation and functional improvement of the contused spinal cord.

摘要

近年来，嗅鞘细胞（OEC）已被用作一种治疗策略，以修复动物和人类受伤脊髓的解剖结构并促进其功能恢复。在这项研究中，将 OEC 移植到成年大鼠的挫伤性脊髓中。在 T10 椎骨行背侧椎板切除术，用 NYU II 撞击器从暴露的脊髓上方 25mm 处施加 10g 的力损伤脊髓。在损伤后 1 周，挫伤性脊髓接受 OEC 在 DMEM 或 DMEM 中的注射。通过光镜观察 OEC 在挫伤性脊髓中的迁移和分布。通过半定量技术在移植后 6 周测量和计数 T8 节段中完整组织区域、损伤组织区域、腔大小、CB-HRP 标记的有髓神经纤维和神经元的数量。通过 BBB 评分和皮质体感诱发电位（CSEP）记录评估脊髓的运动能力和传导功能。在损伤部位和损伤组织附近都发现了 OEC。OEC 移植大鼠的完整组织面积明显大于 DMEM 注射大鼠，而 OEC 大鼠的损伤组织面积明显小于 DMEM 大鼠。病变部位和 T8 节段中保留神经元的有髓神经纤维数量在 OEC 组中明显大于 DMEM 组，但两组之间的腔大小无显著差异。BBB 评分和 CSEP 记录显示，OEC 移植大鼠的运动能力和传导功能表现更好。这些结果表明，OEC 可以对抗脊髓损伤后的继发性组织退化。尽管它们不能减少腔形成，但可以促进挫伤性脊髓的形态保存和功能改善。