一种通过酸化测定未提取人血浆中P物质水平的新方法。

Novel method for determination of substance P levels in unextracted human plasma by using acidification.

作者信息

Campbell Donald E, Bruckner Paula, Tustin Nancy B, Tustin Richard, Douglas Steven D

机构信息

Joseph Stokes Jr. Research Institute at the Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, USA.

出版信息

Clin Vaccine Immunol. 2009 Apr;16(4):594-6. doi: 10.1128/CVI.00406-08. Epub 2009 Feb 4.

DOI:10.1128/CVI.00406-08

PMID:19193832

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2668282/

Abstract

Substance P (SP) is a member of the tachykinin family and has an important role in immune responses. SP is detectable in plasma in a free and bound state. Simple modification of a commercially available SP enzyme-linked immunosorbent assay allows the dissociation and capture of plasma SP without solid-phase extraction.

摘要

P物质（SP）是速激肽家族的一员，在免疫反应中起重要作用。可在血浆中检测到游离和结合状态的P物质。对市售的P物质酶联免疫吸附测定法进行简单修改，无需固相萃取即可解离并捕获血浆中的P物质。