Hansen A, Jørgensen O S, Bolwig T G, Barry D I
Neurobiology Research Group, Rigshospitalet, Copenhagen, Denmark.
J Neurochem. 1991 Nov;57(5):1716-20. doi: 10.1111/j.1471-4159.1991.tb06372.x.
The effect of hippocampal kindling on the regional brain concentration of total glial fibrillary acidic protein (GFAP), a marker of reactive astrocytes, was studied in partially kindled rats, and in fully kindled rats after a post-kindling period of 24 h, 1 week, and 2 months. GFAP concentration was measured in arbitrary units by dot-blots. In the hippocampus, dentate gyrus, basolateral amygdala, pyriform cortex, and entorhinal cortex, limbic structures which are known to be involved in the kindling process, there was an increase in GFAP concentration which was maximal in the fully kindled animals studied after 24 h. In most brain areas, GFAP concentration was still elevated 1 week post-kindling, but had declined to control level 2 months post-kindling. A significant increase in GFAP was also found in septum, ventral pallidum/accumbens nucleus, and primary motor cortex of kindled rats with a post-kindling period of 24 h, whereas in several other brain regions GFAP was unchanged. These results suggest that astrocyte activation, indicative of degenerative changes in nearby neurons, is a transient and regional phenomenon in kindling occurring only during the development of the kindled state.
在部分点燃的大鼠以及点燃后24小时、1周和2个月的完全点燃大鼠中，研究了海马点燃对全胶质纤维酸性蛋白（GFAP，反应性星形胶质细胞的标志物）在脑区浓度的影响。通过斑点印迹法以任意单位测量GFAP浓度。在海马、齿状回、基底外侧杏仁核、梨状皮质和内嗅皮质这些已知参与点燃过程的边缘结构中，GFAP浓度有所增加，在点燃后24小时研究的完全点燃动物中增加最为显著。在大多数脑区，点燃后1周GFAP浓度仍升高，但点燃后2个月已降至对照水平。在点燃后24小时的大鼠的隔区、腹侧苍白球/伏隔核和初级运动皮质中也发现GFAP显著增加，而在其他几个脑区GFAP则无变化。这些结果表明，星形胶质细胞激活，表明附近神经元的退行性变化，是点燃过程中的一种短暂且具有区域特异性的现象，仅在点燃状态发展期间发生。
