Ziegler A, Rockel B, Hegerl R, Freitag B, Lücken U, Plitzko J M
Max-Planck Institute of Biochemistry, Molecular Structural Biology, D-82152 Martinsried, Germany.
J Microsc. 2009 Jan;233(1):170-7. doi: 10.1111/j.1365-2818.2008.03106.x.
This study explores the potential of a C(s)-corrected transmission electron microscope for structural studies of biological samples, in particular isolated macromolecular complexes. A 300-kV transmission electron microscope, equipped with a C(s) corrector was employed to record sets of images at different defocus and C(s) settings. The experiments were designed to determine whether imaging with large defocus benefits from C(s) correction. Defocus contrast in biological imaging has a stronger influence on image resolution than any other parameter. We find the results are in good agreement with theoretical framework, verifying that the typical imaging conditions required for biological investigations are not affected by C(s) correction.
本研究探讨了配备C(s)校正器的透射电子显微镜用于生物样品结构研究的潜力，特别是用于分离的大分子复合物的研究。使用一台配备C(s)校正器的300 kV透射电子显微镜，在不同的欠焦和C(s)设置下记录图像集。这些实验旨在确定大欠焦成像是否受益于C(s)校正。生物成像中的欠焦对比度对图像分辨率的影响比任何其他参数都更强。我们发现结果与理论框架高度一致，证实了生物研究所需的典型成像条件不受C(s)校正的影响。
