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[大鼠肥大细胞细胞化学的组织学特征]

[Organic features of mast cells cytochemistry in rats].

作者信息

Kotsiuba A E, Chertok V M, Kotsiuba E P, Babich E V

出版信息

Tsitologiia. 2008;50(12):1023-9.

Abstract

The mast cells from small intestine mesentery, brain membrane, skin, uterus, wide uterical ligament, ovaries and peritoneal fluid were studied in rats by hystochemical and immunocytochemical technique. Constitutional and inducible NO-synthase, Ca2+-ATPase, cytochromoxidase, and biogenic amines (histamine, dopamine, serotonin) were revealed in mast xells, localized in different tissues. A great number of mast cells with positive cytochromoxidase reaction was presented in skin, intestine mesentery and peritoneal fluid, while the amount of must cells containing biogenic amines was found to be greater in the organs with a significant deal of Ca2+-ATPase and NO-synthase-active cells. The number of degranulating mast cells was lower in the organs with a lack of such cells (skin, wide uterical ligament).

摘要

采用组织化学和免疫细胞化学技术对大鼠小肠系膜、脑膜、皮肤、子宫、阔韧带、卵巢和腹腔液中的肥大细胞进行了研究。在不同组织中的肥大细胞中发现了组成型和诱导型一氧化氮合酶、Ca2+ - ATP酶、细胞色素氧化酶和生物胺(组胺、多巴胺、5 - 羟色胺)。皮肤、小肠系膜和腹腔液中有大量细胞色素氧化酶反应阳性的肥大细胞,而在含有大量Ca2+ - ATP酶和一氧化氮合酶活性细胞的器官中,含有生物胺的肥大细胞数量更多。在缺乏此类细胞的器官(皮肤、阔韧带)中,脱颗粒肥大细胞的数量较少。

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