[朝鲜族中国人群线粒体DNA编码区3954 - 4506多态性]
[Mitochondrial DNA coding region 3954-4506 polymorphism in Korean Chinese population].
作者信息
Zhang Yong-ji, Xu Jing-nan, Zhang Mei-hua, Han Song-ying
机构信息
Department of Forensic Medicine, School of Medicine, Yanbian University, Yanji, Jilin, 133000 PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Feb;26(1):106-8. doi: 10.3760/cma.j.issn.1003-9406.2009.01.025.
OBJECTIVE
To investigate the sequence polymorphism of mtDNA coding region encompassing nt3954-4506 in the Korean Chinese population of Yanbian area.
METHODS
Polymerase chain reaction (PCR) and direct sequencing method were used to detect the haplotype distribution of mtDNA coding region in 198 Korean Chinese individuals.
RESULTS
Twenty-one haplotypes were observed in the 198 unrelated individuals. The genetic diversity was 0.5906 and the random match probability was 0.4124. Compared with the Andersonos sequence, 19 nucleotide variants were found, of which 14 were previously registered in MITOMAP, and 5 were novel.
CONCLUSION
The obtained data suggest that these sequence polymorphisms are valuable genetic markers for personal identification when combined with mtDAN control region investigation, thus could be used as basic data for the forensic application in Korean Chinese population.
目的
研究延边地区朝鲜族汉族人群线粒体DNA（mtDNA）编码区nt3954 - 4506的序列多态性。
方法
采用聚合酶链反应（PCR）和直接测序法检测198例朝鲜族汉族个体mtDNA编码区的单倍型分布。
结果
在198例无关个体中观察到21种单倍型。遗传多样性为0.5906，随机匹配概率为0.4124。与Andersonos序列相比，发现19个核苷酸变异，其中14个先前已在MITOMAP中登记，5个为新发现的。
结论
所获数据表明，这些序列多态性与mtDNA控制区研究相结合时，是用于个人识别的有价值的遗传标记，因此可作为朝鲜族汉族人群法医应用的基础数据。
Zotero 插件