[延边地区中国汉族人群线粒体DNA编码区五个多态性位点]
[Five coding region polymorphism of mitochondrial DNA in Chinese Han population of Yanbian area].
作者信息
Zhang Yongji, Xu Jingnan, Li Zhe
机构信息
Department of Forensic Medicine, Yanbian University School of Medicine, Yanji, Jilin, 133000 PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Dec;26(6):696-700. doi: 10.3760/cma.j.issn.1003-9406.2009.06.019.
OBJECTIVE
To investigate the mitochondrial DNA (mtDNA) five coding region sequence polymorphisms encompassing positions nt3954-4506, nt5218-5974, nt7942-8711, nt10296-10653, and nt14496-14867 in Chinese Han population of Yanbian area, Jilin province.
METHODS
Polymerase chain reaction (PCR) and direct sequencing method were used to detect the haplotype distribution of mtDNA coding region in 200 unrelated Chinese Han individuals.
RESULTS
One hundred and ten haplotypes were observed in the 200 individuals. The gene diversity was 0.9879 and the random match probability was 0.0171. Compared with the Anderson's sequence,81 nucleotide variants were obtained,of which 66 were previously registered in MITOMAP,and 15 were novel.
CONCLUSION
The obtained data suggest that these sequence polymorphisms are valuable genetic markers for personal identification when added to mtDAN control region investigation, and thus could be used as basic data for the forensic application in Chinese Han population.
目的
研究吉林省延边地区中国汉族人群线粒体DNA（mtDNA）五个编码区（nt3954 - 4506、nt5218 - 5974、nt7942 - 8711、nt10296 - 10653和nt14496 - 14867）的序列多态性。
方法
采用聚合酶链反应（PCR）和直接测序法检测200名无血缘关系的中国汉族个体mtDNA编码区的单倍型分布。
结果
在200名个体中观察到110种单倍型。基因多样性为0.9879，随机匹配概率为0.0171。与安德森序列相比，获得了81个核苷酸变异，其中66个先前已在MITOMAP中登记，15个为新发现的。
结论
所得数据表明，这些序列多态性在加入mtDNA控制区研究时是用于个人识别的有价值的遗传标记，因此可作为中国汉族人群法医应用的基础数据。
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