Hanson W L, Chapman W L, Waits V B, Lovelace J K
Department of Parasitology, College of Veterinary Medicine, University of Georgia, Athens 30602.
J Parasitol. 1991 Oct;77(5):780-3.
Young adult (60-70-g) male golden hamsters (Mesocricetus auratus) each were injected intradermally at the dorsal base of the tail with 15 x 10(6) promastigotes of Leishmania (Viannia) panamensis (MHOM/PA/83/WR539), and progression and regression of subsequent lesions were evaluated for up to 17 wk postinfection (PI) as to area, weight, and number of amastigotes within lesions in untreated hamsters and in hamsters treated with meglumine antimoniate (Glucantime). In untreated hamsters total area of lesion, weight, and numbers of amastigotes generally increased rapidly and concomitantly up to 3-4 wk PI. Amastigote numbers tended to decrease from 4 to 11 wk PI and subsequently the numbers of amastigotes within the lesions decreased rapidly, whereas relatively little change occurred in the area and weight of the lesions. Meglumine antimoniate treatment of cutaneous hamster lesions resulted in marked concomitant decrease in size of the lesions and numbers of amastigotes within the lesions examined 1 wk after treatment. Measurement of the area of cutaneous leishmanial lesions thus would appear to be a valid method of evaluating the efficacy of promising compounds against L. panamensis in hamsters when measurements are taken 3-5 wk after experimental infection and reflects the number of amastigotes present in the lesion.
选用年轻成年（60 - 70克）雄性金黄仓鼠（Mesocricetus auratus），每只在其尾背部基部皮内注射15×10⁶ 条巴拿马利什曼原虫（Leishmania (Viannia) panamensis）前鞭毛体（MHOM/PA/83/WR539），对未治疗的仓鼠以及用葡甲胺锑酸盐（Glucantime）治疗的仓鼠，评估感染后长达17周内后续病变的进展和消退情况，包括病变面积、重量以及病变内无鞭毛体数量。在未治疗的仓鼠中，病变总面积、重量和无鞭毛体数量通常在感染后3 - 4周迅速且同步增加。无鞭毛体数量在感染后4至11周趋于减少，随后病变内无鞭毛体数量迅速下降，而病变面积和重量变化相对较小。用葡甲胺锑酸盐治疗仓鼠皮肤病变，在治疗1周后检查发现，病变大小和病变内无鞭毛体数量显著同时减少。因此，当在实验感染后3 - 5周进行测量时，测量皮肤利什曼病病变面积似乎是评估有前景的化合物对仓鼠体内巴拿马利什曼原虫疗效的有效方法，且该方法能反映病变中存在的无鞭毛体数量。
