Davis S W, Speer C A, Dubey J P
Zoonotic Diseases Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
J Parasitol. 1991 Oct;77(5):789-92.
Asexual stages of Sarcocystis neurona were seen in cultured bovine monocytes (M617) inoculated with tissue homogenates from the spinal cord of a horse with naturally acquired protozoal myelitis. Organisms first were observed as intracytoplasmic schizonts and later as motile extracellular zoites capable of infecting surrounding M617 cells. Parasites most often occurred as clusters of merozoites dispersed throughout the host cell cytoplasm; however, schizonts also contained merozoites arranged in a radial fashion surrounding a prominent residual body. Schizonts divided by endopolygeny. The parasite has been maintained beyond 280 days in the laboratory by serial passage of infected M617 cells.
在用自然感染原生动物性脊髓炎的马的脊髓组织匀浆接种的培养牛单核细胞（M617）中观察到了肉孢子虫的无性阶段。最初观察到的生物体为胞内裂殖体，随后为能够感染周围M617细胞的活动胞外子孢子。寄生虫最常表现为分散在整个宿主细胞质中的裂殖子簇；然而，裂殖体也含有围绕一个突出的残体呈放射状排列的裂殖子。裂殖体通过内多殖进行分裂。通过连续传代感染的M617细胞，该寄生虫已在实验室中维持了280天以上。
